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Analytical validation of quantitative real-time PCR methods for quantification of trypanosoma cruzi DNA in blood samples from chagas disease patients

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dc.creatorRamírez, Juan Carlosspa
dc.creatorCura, Carolina Inésspa
dc.creatorda Cruz Moreira, Otaciliospa
dc.creatorLages-Silva, Elianespa
dc.creatorJuiz,Nataliaspa
dc.date.accessioned2020-08-06T16:20:57Z
dc.date.available2020-08-06T16:20:57Z
dc.date.created2015-09-01spa
dc.description.abstractAn international study was performed by 26 experienced PCR laboratories from 14 countries to assess the performance of duplex quantitative real-time PCR (qPCR) strategies on the basis of TaqMan probes for detection and quantification of parasitic loads in peripheral blood samples from Chagas disease patients. Two methods were studied: Satellite DNA (SatDNA) qPCR and kinetoplastid DNA (kDNA) qPCR. Both methods included an internal amplification control. Reng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.jmoldx.2015.04.010
dc.identifier.issnISSN: 1525-1578
dc.identifier.issnEISSN: 1943-7811
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/26203
dc.language.isoengspa
dc.publisherAmerican Society for Investigative Pathology and the Association for Molecular Pathologyspa
dc.publisherElsevierspa
dc.relation.citationEndPage615
dc.relation.citationIssueNo. 5
dc.relation.citationStartPage605
dc.relation.citationTitleThe Journal of Molecular Diagnostics
dc.relation.citationVolumeVol. 17
dc.relation.ispartofThe Journal of Molecular Diagnostics, ISSN: 1525-1578;EISSN: 1943-7811 Vol.17, No.5 (2015); pp.605-615spa
dc.relation.urihttps://jmd.amjpathol.org/action/showPdf?pii=S1525-1578%2815%2900129-4spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourceThe Journal of Molecular Diagnosticsspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordAnalytical validationspa
dc.subject.keywordDNAspa
dc.subject.keywordTrypanosoma rangelispa
dc.subject.keywordLeishmania sppspa
dc.titleAnalytical validation of quantitative real-time PCR methods for quantification of trypanosoma cruzi DNA in blood samples from chagas disease patientsspa
dc.title.TranslatedTitleValidación analítica de métodos cuantitativos de PCR en tiempo real para la cuantificación del ADN de Trypanosoma cruzi en muestras de sangre de pacientes con enfermedad de Chagasspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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