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Contemporary cryptic sexuality in Trypanosoma cruzi

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dc.creatorRamírez, Juan Davidspa
dc.creatorGuhl, Felipespa
dc.creatorMessenger, Louisa A.spa
dc.creatorLewis, Michael D.spa
dc.creatorMontilla, Marlenyspa
dc.creatorCucunuba, Zulmaspa
dc.creatorMiles, Michael A.spa
dc.creatorLlewellyn, Martin S.spa
dc.date.accessioned2020-08-19T14:42:08Z
dc.date.available2020-08-19T14:42:08Z
dc.date.created2012-07-09spa
dc.description.abstractClonal propagation is considered to be the predominant mode of reproduction among many parasitic protozoa. However, this assumption may overlook unorthodox, infrequent or cryptic sexuality. Trypanosoma cruzi , which causes Chagas disease, is known to undergo non?Mendelian genetic exchange in the laboratory. In the field, evidence of extant genetic exchange is limited. In this study, we undertook intensive sampling of T. cruzi Discrete Typing Unit I in endemic eastern Colombia. Using Fluorescence?activated cell sorting, we generated 269 biological clones from 67 strains. Each clone was genotyped across 24 microsatellite loci. Subsequently, 100 representative clones were typed using 10 mitochondrial sequence targets (3.76?Kbp total). Clonal diversity among humans, reservoir hosts and vectors suggested complex patterns of superinfection and/or coinfection in oral and vector?borne Chagas disease cases. Clonal diversity between mother and foetus in a congenital case demonstrates that domestic TcI genotypes are infective in utero . Importantly, gross incongruence between nuclear and mitochondrial markers is strong evidence for widespread genetic exchange throughout the data set. Furthermore, a confirmed mosaic maxicircle sequence suggests intermolecular recombination between individuals as a further mechanism of genetic reassortment. Finally, robust dating based on mitochondrial DNA indicates that the emergence of a widespread domestic TcI clade that we now name TcIDOM (formerly TcIa/VENDom) occurred 23?000?±?12?000?years ago and was followed by population expansion, broadly corresponding with the earliest human migration into the Americas.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/j.1365-294X.2012.05699.x
dc.identifier.issnISSN: 0962-1083
dc.identifier.issnEISSN: 1365-294X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/27423
dc.language.isoengspa
dc.publisherJohn Wiley & Sonsspa
dc.relation.citationEndPage4226
dc.relation.citationIssueNo. 17
dc.relation.citationStartPage4216
dc.relation.citationTitleMolecular Ecology
dc.relation.citationVolumeVol. 21
dc.relation.ispartofMolecular Ecology, ISSN: 0962-1083;EISSN: 1365-294X, Vol.21, No.17 (September 2012); pp. 4216-4226spa
dc.relation.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-294X.2012.05699.xspa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceMolecular Ecologyspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordDisease biologyspa
dc.subject.keywordEmpiricalspa
dc.subject.keywordEvolution of sexspa
dc.subject.keywordMolecular evolutionspa
dc.subject.keywordParasitologyspa
dc.subject.keywordPopulation geneticsspa
dc.subject.keywordProtistsspa
dc.titleContemporary cryptic sexuality in Trypanosoma cruzispa
dc.title.TranslatedTitleSexualidad críptica contemporánea en Trypanosoma cruzispa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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