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Blastocystis and urticaria: Examination of subtypes and morphotypes in an unusual clinical manifestation

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dc.creatorCasero, Rodolfo Danielspa
dc.creatorMongi, Florenciaspa
dc.creatorSánchez, Angiespa
dc.creatorRamírez, Juan David
dc.date.accessioned2020-05-26T00:03:48Z
dc.date.available2020-05-26T00:03:48Z
dc.date.created2015spa
dc.description.abstractBlastocystis is a human common enteric protist that may colonize a large variety of non-human hosts linked to symptoms and diseases such as abdominal pain, constipation, diarrhea, urticaria, flatulence and irritable bowel syndrome (IBS). Blastocystis exhibits remarkable genetic diversity and multiple subtypes (STs) within the genus with no absolute associations with clinical symptomatology. Here we analyzed fecal samples from Argentinean patients (. n=. 270) belonging to symptomatic (urticaria and non-specific gastrointestinal symptoms, n=. 39) and asymptomatic control (. n=. 28). Those patients infected with Blastocystis (. n=. 67) were submitted for morphological analysis, DNA extraction, 18S PCR, sequencing and STs identification according to DNA barcoding. Blastocystis vacuolar forms were the predominant morphotype (75%), ameboid-like forms were evidenced in 1.5% of samples. Blastocystis ST3 was detected in 71.6% (. n=. 48), of which 71.4%, (. n=. 35) and 28.6% (. n=. 14) belonged to symptomatic and asymptomatic respectively. Other subtypes identified were ST1 (14.9%), ST6 (7.5%) and ST2 (5.9%). Blastocystis 18S barcoding evidenced in non-urticaria symptomatic patients and asymptomatic control group the presence of allele 134 (ST3) (. p less than . 0.0001), while allele 34 (ST3) was detected in 85.7% (18/21) of symptomatic uricaria as compared with control group (1/21) (. p less than . 0.0001). The presence of a particular allele (. a34) significantly associated with urticaria patients was detected and the clinical implications of these findings are herein discussed. © 2015.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.actatropica.2015.05.004
dc.identifier.issn0001706X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23630
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage161
dc.relation.citationStartPage156
dc.relation.citationTitleActa Tropica
dc.relation.citationVolumeVol. 148
dc.relation.ispartofActa Tropica, ISSN:0001706X, Vol.148,(2015); pp. 156-161spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84929396178&doi=10.1016%2fj.actatropica.2015.05.004&partnerID=40&md5=45fae3bfa5179d6290466d5bfc573316spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordDnaspa
dc.subject.keywordRna 18sspa
dc.subject.keywordAllelespa
dc.subject.keywordDnaspa
dc.subject.keywordMorphologyspa
dc.subject.keywordMorphotypespa
dc.subject.keywordParasitespa
dc.subject.keywordProtistspa
dc.subject.keywordProtozoanspa
dc.subject.keywordAdolescentspa
dc.subject.keywordAdultspa
dc.subject.keywordAllelespa
dc.subject.keywordArgentinianspa
dc.subject.keywordArticlespa
dc.subject.keywordAsymptomatic diseasespa
dc.subject.keywordBlastocystisspa
dc.subject.keywordBlastocystosisspa
dc.subject.keywordChildspa
dc.subject.keywordControlled studyspa
dc.subject.keywordDna barcodingspa
dc.subject.keywordDna extractionspa
dc.subject.keywordFemalespa
dc.subject.keywordGastrointestinal symptomspa
dc.subject.keywordGene sequencespa
dc.subject.keywordHumanspa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordMalespa
dc.subject.keywordMorphotypespa
dc.subject.keywordNonhumanspa
dc.subject.keywordParasite identificationspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordUrticariaspa
dc.subject.keywordAbdominal painspa
dc.subject.keywordArgentinaspa
dc.subject.keywordAsymptomatic infectionspa
dc.subject.keywordBlastocystisspa
dc.subject.keywordBlastocystis infectionsspa
dc.subject.keywordClassificationspa
dc.subject.keywordConstipationspa
dc.subject.keywordDiarrheaspa
dc.subject.keywordDna sequencespa
dc.subject.keywordFecesspa
dc.subject.keywordGenetic variabilityspa
dc.subject.keywordGeneticsspa
dc.subject.keywordInfantspa
dc.subject.keywordMiddle agedspa
dc.subject.keywordParasitologyspa
dc.subject.keywordPreschool childspa
dc.subject.keywordUrticariaspa
dc.subject.keywordYoung adultspa
dc.subject.keywordBlastocystisspa
dc.subject.keywordProtistaspa
dc.subject.keywordAbdominal painspa
dc.subject.keywordAdolescentspa
dc.subject.keywordAdultspa
dc.subject.keywordAllelesspa
dc.subject.keywordArgentinaspa
dc.subject.keywordAsymptomatic infectionsspa
dc.subject.keywordBlastocystisspa
dc.subject.keywordBlastocystis infectionsspa
dc.subject.keywordChildspa
dc.subject.keywordChildeng
dc.subject.keywordConstipationspa
dc.subject.keywordDiarrheaspa
dc.subject.keywordFecesspa
dc.subject.keywordFemalespa
dc.subject.keywordGenetic variationspa
dc.subject.keywordHumansspa
dc.subject.keywordInfantspa
dc.subject.keywordMalespa
dc.subject.keywordMiddle agedspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordRna, ribosomal, 18sspa
dc.subject.keywordSequence analysiseng
dc.subject.keywordUrticariaspa
dc.subject.keywordYoung adultspa
dc.subject.keywordBlastocystis st3 allele 34 and urticariaspa
dc.subject.keywordBlastocystis subtypesspa
dc.subject.keywordDna barcodingspa
dc.subject.keywordUrticariaspa
dc.titleBlastocystis and urticaria: Examination of subtypes and morphotypes in an unusual clinical manifestationspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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