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In Vitro Antifungal Activity of Three Synthetic Peptides against Candida auris and Other Candida Species of Medical Importance

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dc.creatorTorres, Richarspa
dc.creatorBarreto-Santamaría, Adrianaspa
dc.creatorArévalo-Pinzón, Gabrielaspa
dc.creatorFiracative, Carolinaspa
dc.creatorGómez, Beatriz L.spa
dc.creatorEscandón, Patriciaspa
dc.creatorPatarroyo, Manuel Alfonsospa
dc.creatorMuñoz Henao, Julián Estebanspa
dc.date.accessioned2024-01-31T18:27:09Z
dc.date.available2024-01-31T18:27:09Z
dc.date.created2023-08-01spa
dc.date.issued2023spa
dc.descriptionCandidiasis is an opportunistic infection affecting immunosuppressed and hospitalized patients, with mortality rates approaching 40% in Colombia. The growing pharmacological resistance of Candida species and the emergence of multidrug-resistant Candida auris are major public health problems. Therefore, different antimicrobial peptides (AMPs) are being investigated as therapeutic alternatives to control candidiasis effectively and safely. This work aimed to evaluate the in vitro antifungal activity of three synthetic AMPs, PNR20, PNR20-1, and 35409, against ATCC reference strains of Candida albicans, Candida glabrata, Candida parapsilosis, Candida krusei, and Candida tropicalis, and clinical isolates of C. auris. Antifungal susceptibility testing, determined by broth microdilution, showed that the AMPs have antifungal activity against planktonic cells of all Candida species evaluated. In C. auris and C. albicans, the peptides had an effect on biofilm formation and cell viability, as determined by the XTT assay and flow cytometry, respectively. Also, morphological alterations in the membrane and at the intracellular level of these species were induced by the peptides, as observed by transmission electron microscopy. In vitro, the AMPs had no cytotoxicity against L929 murine fibroblasts. Our results showed that the evaluated AMPs are potential therapeutic alternatives against the most important Candida species in Colombia and the world.spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttp://doi.org/10.3390/antibiotics12081234spa
dc.identifier.issn2079-6382spa
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/42130
dc.language.isoengspa
dc.publisherUniversidad del Rosariospa
dc.relation.urihttps://www.mdpi.com/2079-6382/12/8/1234spa
dc.rightsAttribution 4.0 Internationalspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/spa
dc.sourceAntibioticsspa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectCandida aurisspa
dc.subjectCandida spp.spa
dc.subjectantimicrobial peptidesspa
dc.subjectantifungal resistancespa
dc.subjectcandidiasisspa
dc.titleIn Vitro Antifungal Activity of Three Synthetic Peptides against Candida auris and Other Candida Species of Medical Importancespa
dc.typearticlespa
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.type.spaArtículospa
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