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Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)

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dc.creatorSegura, Nidya Aspa
dc.creatorSantamaría, Erikaspa
dc.creatorCabrera, Olga Lspa
dc.creatorBello, Feliospa
dc.date.accessioned2020-05-25T23:57:00Z
dc.date.available2020-05-25T23:57:00Z
dc.date.created2012spa
dc.description.abstractInsect cell cultures are an important biotechnological tool for basic and applied studies. The objective of this work was to establish and characterise a new cell line from Culex quinquefasciatus embryonic tissues. Embryonated eggs were taken as a source of tissue to make explants that were seeded in L-15, Grace's, Grace's/L-15, MM/VP12, Schneider's and DMEM culture media with a pH range from 6.7-6.9 and incubated at 28°C. The morphological, cytogenetic, biochemical and molecular characteristics of the cell cultures were examined by observing the cell shapes, obtaining the karyotypes, using a cellulose-acetate electrophoretic system and performing random amplified polymorphic DNA-polymerase chain reaction analysis, respectively. The Grace's/L-15 medium provided the optimal nutritional conditions for cell adhesion and proliferation. Approximately 40-60 days following the explant procedure, a confluent monolayer was formed. Cellular morphology in the primary cultures and the subcultures was heterogeneous, but in the monolayer the epithelioid morphology type predominated. A karyotype with a diploid number of six chromosomes (2n = 6) was observed. Isoenzymatic and molecular patterns of the mosquito cell cultures matched those obtained from the immature and adult forms of the same species. Eighteen subcultures were generated. These cell cultures potentially constitute a useful tool for use in biomedical applications.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1590/S0074-02762012000100013
dc.identifier.issn16788060
dc.identifier.issn00740276
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22579
dc.language.isoengspa
dc.publisherFundacao Oswaldo Cruzspa
dc.relation.citationEndPage95
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage89
dc.relation.citationTitleMemorias do Instituto Oswaldo Cruz
dc.relation.citationVolumeVol. 107
dc.relation.ispartofMemorias do Instituto Oswaldo Cruz, ISSN:16788060, 00740276, Vol.107, No.1 (2012); pp. 89-95spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84857099648&doi=10.1590%2fS0074-02762012000100013&partnerID=40&md5=95cc2f5423366c796bea1f24c5e7dfa2spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordCulex pipiens quinquefasciatusspa
dc.subject.keywordCulicidaespa
dc.subject.keywordDipteraspa
dc.subject.keywordHexapodaspa
dc.subject.keywordCulture mediaspa
dc.subject.keywordEmbryonic tissuesspa
dc.subject.keywordIsoenzymatic patternsspa
dc.subject.keywordKaryotypespa
dc.subject.keywordRAPD-PCRspa
dc.titleEstablishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)spa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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