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Glucosylceramides from Lomentospora prolificans induce a differential production of cytokines and increases the microbicidal activity of macrophages

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dc.creatorda Silva Xisto, Mariana Ingrid Dutraspa
dc.creatorMuñoz Henao, Julián Estebanspa
dc.creatordos Santos Dias, Lucasspa
dc.creatorSantos, Giulia Maria Piresspa
dc.creatorde Oliveira Rocha Calixto, Renataspa
dc.creatorBernardino, Mariana Collodettispa
dc.creatorTaborda, Carlos Pelleschispa
dc.creatorBarreto-Bergter, Elianaspa
dc.date.accessioned2020-05-26T00:08:32Z
dc.date.available2020-05-26T00:08:32Z
dc.date.created2019spa
dc.description.abstractLomentospora prolificans is an emerging opportunistic fungus with a high resistance to antifungal agents and it can cause localized infections in immunocompetent patients and disseminated infections with a high mortality rate in immunosuppressed patients. Glucosylceramides (GlcCer) are synthetized in the majority of known fungal pathogens. They are bioactive molecules presenting different functions, such as involvement in fungal growth and morphological transitions in several fungi. The elucidation of the primary structure of the fungal surface glycoconjugates could contribute for the understanding of the mechanisms of pathogenicity. In this work, GlcCer species were isolated from mycelium and conidia forms of L. prolificans and their chemical structures were elucidated by mass spectrometry (ESI-MS). GlcCer purified from both forms presented a major species at m/z 750 that corresponds to N-2-hydroxyhexadecanoyl-1-?-D-glucopyranosyl-9-methyl-4,8-sphingadienine. Monoclonal antibodies against GlcCer could recognize L. prolificans GlcCer species from mycelium and conidia, suggesting a conserved epitope in fungal GlcCer. In addition, in vivo assays showed that purified GlcCer species from both forms was able to induce a high secretion of pro-inflammatory cytokines by splenocytes. GlcCer species also promote the recruitment of polymorphonuclear, eosinophils, small peritoneal macrophage (SPM) and mononuclear cells to the peritoneal cavity. GlcCer species were also able to induce the oxidative burst by peritoneal macrophages with NO and superoxide radicals production, and to increase the killing of L. prolificans conidia by peritoneal macrophages. These results indicate that GlcCer species from L. prolificans are a potent immune response activator. Copyright © 2019 Xisto, Henao, Dias, Santos, Calixto, Bernardino, Taborda and Barreto-Bergter.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3389/fmicb.2019.00554
dc.identifier.issn1664302X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24092
dc.language.isoengspa
dc.publisherFrontiers Media S.A.spa
dc.relation.citationIssueNo. MAR
dc.relation.citationTitleFrontiers in Microbiology
dc.relation.citationVolumeVol. 10
dc.relation.ispartofFrontiers in Microbiology, ISSN:1664302X, Vol.10, No.MAR (2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85066436307&doi=10.3389%2ffmicb.2019.00554&partnerID=40&md5=cd6dcceb4294a7516a3e297b7e14ec89spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordCXCL2 chemokinespa
dc.subject.keywordGamma interferonspa
dc.subject.keywordGlucosylceramidespa
dc.subject.keywordInterleukin 10spa
dc.subject.keywordInterleukin 17spa
dc.subject.keywordSuperoxidespa
dc.subject.keywordTumor necrosis factorspa
dc.subject.keywordAnimal experimentspa
dc.subject.keywordAnimal modelspa
dc.subject.keywordAntigen recognitionspa
dc.subject.keywordAntioxidant activityspa
dc.subject.keywordArticlespa
dc.subject.keywordCarbohydrate analysisspa
dc.subject.keywordCellular distributionspa
dc.subject.keywordCytokine productionspa
dc.subject.keywordElectrospray mass spectrometryspa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordFemalespa
dc.subject.keywordFungus growthspa
dc.subject.keywordHigh performance thin layer chromatographyspa
dc.subject.keywordImmune responsespa
dc.subject.keywordImmunocompetencespa
dc.subject.keywordImmunocompromised patientspa
dc.subject.keywordImmunodetectionspa
dc.subject.keywordImmunofluorescencespa
dc.subject.keywordImmunohistochemistryspa
dc.subject.keywordImmunoreactivityspa
dc.subject.keywordMacrophagespa
dc.subject.keywordMicrobicidal activityspa
dc.subject.keywordMortality ratespa
dc.subject.keywordMousespa
dc.subject.keywordNonhumanspa
dc.subject.keywordPathogenicityspa
dc.subject.keywordPhagocytosisspa
dc.subject.keywordRespiratory burstspa
dc.subject.keywordGlccerspa
dc.subject.keywordGlucosylceramidesspa
dc.subject.keywordInnate immune responsespa
dc.subject.keywordLomentospora prolificansspa
dc.subject.keywordStructural characterizationspa
dc.titleGlucosylceramides from Lomentospora prolificans induce a differential production of cytokines and increases the microbicidal activity of macrophagesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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