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Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines

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dc.creatorGarcía, Javierspa
dc.creatorPuentes, Alvarospa
dc.creatorRodríguez, Luis
dc.creatorOcampo, Marisol
dc.creatorCurtidor, Hernandospa
dc.creatorVera, Ricardospa
dc.date.accessioned2020-08-19T14:39:54Z
dc.date.available2020-08-19T14:39:54Z
dc.date.created2005-09spa
dc.description.abstractThe gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in the Mycobacterium tuberculosis complex (as assayed by PCR) and transcribed (as determined by RT?PCR) in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum strains. Rabbits immunized with synthetic polymer peptides from this protein produced antibodies specifically recognizing a 25?kDa band in mycobacterial sonicate. U937 and A549 cells were used in binding assays involving 20?amino?acid?long synthetic peptides covering the whole Rv2536 protein sequence. Peptide 11207 (161DVFSAVRADDSPTGEMQVAQY180) presented high specific binding to both types of cells; the binding was saturable and presented nanomolar affinity constants. Cross?linking assays revealed that this peptide specifically binds to 50 kDa U937 cell membrane and 45 kDa A549 cell membrane proteins.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1110/ps.051526305
dc.identifier.issnISSN: 0961-8368
dc.identifier.issnEISSN: 1469-896X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/26623
dc.language.isoengspa
dc.publisherThe Protein Societyspa
dc.publisherJohn Wiley & Sonspa
dc.relation.citationEndPage2245
dc.relation.citationIssueNo. 9
dc.relation.citationStartPage2236
dc.relation.citationTitleProtein Science
dc.relation.citationVolumeVol. 14
dc.relation.ispartofProtein Science, ISSN: 0961-8368;EISSN: 1469-896X, Vol.14, No.9 (September 2005); pp. 2236-2245spa
dc.relation.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1110/ps.051526305spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourceProtein Sciencespa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordHigh activity binding peptidesspa
dc.subject.keywordReceptor–ligand interactionspa
dc.subject.keywordMycobacterium tuberculosis complexspa
dc.subject.keywordHABPspa
dc.subject.keywordhigh activity binding peptidespa
dc.subject.keywordBS3spa
dc.subject.keywordbis?sulfosuccinimidyl suberatespa
dc.subject.keywordHBSspa
dc.subject.keywordhepes buffer salinespa
dc.subject.keywordSEC size exclusion chromatographyspa
dc.titleMycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell linesspa
dc.title.TranslatedTitleProteína de Mycobacterium tuberculosis Rv2536 implicada en la unión específica a líneas celulares humanasspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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