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Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina

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dc.creatorCasero R.D.spa
dc.creatorMongi F.spa
dc.creatorLaconte L.spa
dc.creatorRivero F.spa
dc.creatorSastre D.spa
dc.creatorTeherán A.spa
dc.creatorHerrera G.spa
dc.creatorRamírez, Juan Davidspa
dc.date.accessioned2020-05-26T00:11:28Z
dc.date.available2020-05-26T00:11:28Z
dc.date.created2017spa
dc.description.abstractIn this study, we describe the frequency of Acanthamoeba keratitis (AK) in patients that assisted in the Ophthalmology Department and determine the species/genotypes of free living amoebas (FLA) isolates. FLA from Corneal scrapes (CS) and contact lens (CL) wearers were studied by morphological and molecular characterization. A database was constructed with sociodemographic, clinical findings and history of use of CL variables. During January 2000 and September 2016 patients with corneal pathology admitted to the Ophthalmology Service of the University Hospital in Córdoba city, Argentina were included in the study. FLA were detected in 1.5% (11/739) and in 17% (11/65) of CS and CL analyzed respectively. FLA isolates from CL users evidenced an 80.9% of inappropriate lens maintenance, 4.8% (1/21) were not CL users that have been in contact with waters in outdoor environment and 14,3% (3/21) with no data about CL users. Acanthamoeba was confirmed in 100% and 82% of CS and LC respectively. The most frequent symptom associated with AK was red eye and photophobia. FLA from CS belonged to group II but 82% (9/11) and 18% (2/11) from CL belonged to group II and III respectively. T4 genotype and A. polyphaga species were detected in 100% of Acanthamoeba isolates. Poor CL hygiene practices, highlights the need for improved education about the severity of AK and consequences of improper CL hygiene. Genotype T4 detected in 100% of both CS and CL samples, consistently with previous findings indicating that this genotype is by far the most prevalent isolated from ocular infection. © 2017 Elsevier B.V.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.meegid.2017.06.031
dc.identifier.issn15671348
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24304
dc.language.isoengspa
dc.publisherElsevier B.V.spa
dc.relation.citationEndPage175
dc.relation.citationStartPage170
dc.relation.citationTitleInfection, Genetics and Evolution
dc.relation.citationVolumeVol. 54
dc.relation.ispartofInfection, Genetics and Evolution, ISSN:15671348, Vol.54,(2017); pp. 170-175spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85021950076&doi=10.1016%2fj.meegid.2017.06.031&partnerID=40&md5=598b0f96a6d0b51ab656bf83cdcbd1d5spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAcanthamoeba keratitisspa
dc.subject.keywordAcanthamoeba polyphagaspa
dc.subject.keywordAdultspa
dc.subject.keywordAgedspa
dc.subject.keywordArgentinaspa
dc.subject.keywordArticlespa
dc.subject.keywordContact lensspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCorneaspa
dc.subject.keywordDemographyspa
dc.subject.keywordDisease associationspa
dc.subject.keywordDisease severityspa
dc.subject.keywordEnvironmental factorspa
dc.subject.keywordEye rednessspa
dc.subject.keywordFemalespa
dc.subject.keywordGenotype phenotype correlationspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman tissuespa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordMalespa
dc.subject.keywordMorphologyspa
dc.subject.keywordNonhumanspa
dc.subject.keywordParasite identificationspa
dc.subject.keywordParasite isolationspa
dc.subject.keywordPhotophobiaspa
dc.subject.keywordPhylogenetic treespa
dc.subject.keywordPriority journalspa
dc.subject.keywordSequence analysisspa
dc.subject.keywordSocial statusspa
dc.subject.keywordAcanthamoebaspa
dc.subject.keywordAcanthamoeba keratitisspa
dc.subject.keywordClassificationspa
dc.subject.keywordContact lensspa
dc.subject.keywordGeneticsspa
dc.subject.keywordGenotypespa
dc.subject.keywordMiddle agedspa
dc.subject.keywordParasite loadspa
dc.subject.keywordParasitologyspa
dc.subject.keywordPhylogenyspa
dc.subject.keywordVery elderlyspa
dc.subject.keywordAcanthamoebaspa
dc.subject.keywordAcanthamoeba Keratitisspa
dc.subject.keywordAdultspa
dc.subject.keywordAgedspa
dc.subject.keywordAged, 80 and overspa
dc.subject.keywordArgentinaspa
dc.subject.keywordContact Lensesspa
dc.subject.keywordFemalespa
dc.subject.keywordGenotypespa
dc.subject.keywordHumansspa
dc.subject.keywordMalespa
dc.subject.keywordMiddle Agedspa
dc.subject.keywordParasite Loadspa
dc.subject.keywordPhylogenyspa
dc.subject.keywordAcanthamoebaspa
dc.subject.keywordArgentinaspa
dc.subject.keywordContact lensspa
dc.subject.keywordGenotypespa
dc.subject.keywordKeratitisspa
dc.titleMolecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentinaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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