Ítem
Acceso Abierto

Acute hydrodynamic damage induced by SPLITT fractionation and centrifugation in red blood cells

Mostrar el registro sencillo de la publicación
dc.creatorUrbina Bonilla, Adriana del Pilar
dc.creatorGodoy-Silva, Rubenspa
dc.creatorHoyos, Mauriciospa
dc.creatorCamacho, Marcelaspa
dc.date.accessioned2020-05-25T23:56:00Z
dc.date.available2020-05-25T23:56:00Z
dc.date.created2016spa
dc.description.abstractThough blood bank processing traditionally employs centrifugation, new separation techniques may be appealing for large scale processes. Split-flow fractionation (SPLITT) is a family of techniques that separates in absence of labelling and uses very low flow rates and force fields, and is therefore expected to minimize cell damage. However, the hydrodynamic stress and possible consequent damaging effects of SPLITT fractionation have not been yet examined. The aim of this study was to investigate the hydrodynamic damage of SPLITT fractionation to human red blood cells, and to compare these effects with those induced by centrifugation. Peripheral whole blood samples were collected from healthy volunteers. Samples were diluted in a buffered saline solution, and were exposed to SPLITT fractionation (flow rates 1-10 ml/min) or centrifugation (100-1500 g) for 10 min. Cell viability, shape, diameter, mean corpuscular hemoglobin, and membrane potential were measured. Under the operating conditions employed, both SPLITT and centrifugation maintained cell viability above 98%, but resulted in significant sublethal damage, including echinocyte formation, decreased cell diameter, decreased mean corpuscular hemoglobin, and membrane hyperpolarization which was inhibited by EGTA. Wall shear stress and maximum energy dissipation rate showed significant correlation with lethal and sublethal damage. Our data do not support the assumption that SPLITT fractionation induces very low shear stress and is innocuous to cell function. Some changes in SPLITT channel design are suggested to minimize cell damage. Measurement of membrane potential and cell diameter could provide a new, reliable and convenient basis for evaluation of hydrodynamic effects on different cell models, allowing identification of optimal operating conditions on different scales. © 2016 Elsevier B.V.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.jchromb.2016.03.025
dc.identifier.issn15700232
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22288
dc.language.isoengspa
dc.publisherElsevier B.V.spa
dc.relation.citationEndPage61
dc.relation.citationStartPage53
dc.relation.citationTitleJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
dc.relation.citationVolumeVol. 1020
dc.relation.ispartofJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, ISSN:15700232, Vol.1020,(2016); pp. 53-61spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84961626327&doi=10.1016%2fj.jchromb.2016.03.025&partnerID=40&md5=0927b094400b56f8c8fa23b1e63b29efspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordBloodspa
dc.subject.keywordCentrifugationspa
dc.subject.keywordCytologyspa
dc.subject.keywordEnergy dissipationspa
dc.subject.keywordFluid dynamicsspa
dc.subject.keywordHemoglobinspa
dc.subject.keywordHydrodynamicsspa
dc.subject.keywordShear stressspa
dc.subject.keywordEnergy dissipation ratespa
dc.subject.keywordHuman red blood cellspa
dc.subject.keywordMembrane potentialsspa
dc.subject.keywordOperating conditionspa
dc.subject.keywordOptimal operating conditionsspa
dc.subject.keywordRed blood cellspa
dc.subject.keywordSeparation techniquesspa
dc.subject.keywordSPLITT fractionationspa
dc.subject.keywordCellsspa
dc.subject.keywordEgtazic acidspa
dc.subject.keywordSodium chloridespa
dc.subject.keywordArticlespa
dc.subject.keywordCell damagespa
dc.subject.keywordCell functionspa
dc.subject.keywordCell shapespa
dc.subject.keywordCell structurespa
dc.subject.keywordCell viabilityspa
dc.subject.keywordCentrifugationspa
dc.subject.keywordComparative studyspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCorrelation analysisspa
dc.subject.keywordEchinocytespa
dc.subject.keywordErythrocytespa
dc.subject.keywordFractionationspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordHydrodynamicsspa
dc.subject.keywordHyperpolarizationspa
dc.subject.keywordMean corpuscular hemoglobinspa
dc.subject.keywordMembrane potentialspa
dc.subject.keywordPriority journalspa
dc.subject.keywordShear stressspa
dc.subject.keywordSplit flow fractionationspa
dc.subject.keywordAdverse effectsspa
dc.subject.keywordBiomechanicsspa
dc.subject.keywordCell separationspa
dc.subject.keywordCell survivalspa
dc.subject.keywordCentrifugationspa
dc.subject.keywordCytologyspa
dc.subject.keywordDevicesspa
dc.subject.keywordEquipment designspa
dc.subject.keywordErythrocytespa
dc.subject.keywordErythrocyte membranespa
dc.subject.keywordHydrodynamicsspa
dc.subject.keywordPhysiologyspa
dc.subject.keywordProceduresspa
dc.subject.keywordShear strengthspa
dc.subject.keywordBiomechanical Phenomenaspa
dc.subject.keywordCell Separationspa
dc.subject.keywordCell Shapespa
dc.subject.keywordCell Survivalspa
dc.subject.keywordCentrifugationspa
dc.subject.keywordEquipment Designspa
dc.subject.keywordErythrocyte Membranespa
dc.subject.keywordErythrocytesspa
dc.subject.keywordHumansspa
dc.subject.keywordHydrodynamicsspa
dc.subject.keywordMembrane Potentialsspa
dc.subject.keywordShear Strengthspa
dc.subject.keywordCentrifugationspa
dc.subject.keywordEnergy dissipation ratespa
dc.subject.keywordHydrodynamic damagespa
dc.subject.keywordRed blood cellsspa
dc.subject.keywordSPLITT fractionationspa
dc.titleAcute hydrodynamic damage induced by SPLITT fractionation and centrifugation in red blood cellsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
1-s2-0-S157002321630174X-main.pdf
Tamaño:
1.38 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos