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Caracterización genómica de especies de Clostridiales Circulantes en el río Pasto

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dc.contributor.advisorMuñoz Díaz, Marina
dc.contributor.gruplacGrupo de Investigaciones Microbiológicas UR (GIMUR)
dc.creatorFernández Sánchez, Juan Diego
dc.creator.degreeBiólogo
dc.creator.degreeLevelPregrado
dc.creator.degreetypeFull time
dc.date.accessioned2024-09-03T20:58:06Z
dc.date.available2024-09-03T20:58:06Z
dc.date.created2024-08-22
dc.descriptionLa clase Clostridia está compuesta por bacterias con diversos roles potenciales, contando con especies comensales y otras patógenos oportunistas. Estas bacterias son clasificadas como anaerobias y están presentes a nivel intestinal de humanos y animales; sin embargo, la alta resistencia de sus esporas al oxígeno les permite ampliar su rango de dispersión, siendo encontrados en muestras ambientales de suelos y aguas. La presencia de este tipo de microorganismos en cuerpos de agua puede ser un marcador de pérdida de su calidad. Zonas en las que es frecuente el contacto entre poblaciones humanas y cuerpos hídricos pueden ser propensas a contaminación de diferentes tipos, siendo una de ellas los microorganismos, muchos de los cuales son de importancia clínica por su potencial patógeno. Este estudio tuvo como objetivo detectar y caracterizar a nivel genómico bacterias Clostridiales aisladas de muestras de agua, tomadas entre septiembre del 2022 hasta marzo del 2023 del Río Pasto. Para el proceso de caracterización se ensamblaron Genomas Metagenómicos Ensamblados (MAGs) en los cuales se identificaron marcadores de resistencia a antimicrobianos y factores de virulencia, siendo esto tema relevante de salud pública. Se ensamblaron 33 MAGs de alta calidad y cuatro de calidad media, correspondientes a 11 especies clostridiales, estando entre estas Clostridium perfringens, Clostridium tetani, Clostridium botulinum y Clostridioides difficile, que son medicamente relevantes. Pymaiobacter missiliensis y Clostridium sulfidigenes también fueron detectadas en este estudio, siendo especies Clostridiales recientemente descritas y poco estudiadas, por lo que no es claro su impacto sobre hospederos. La búsqueda de factores de virulencia a partir de los MAGs mostró la presencia de genes nag, junto con otros genes de este tipo frecuentes en C. perfringens. Adicionalmente, se encontraron marcadores de resistencia a antibióticos, especialmente asociados a resistencia a fluoroquinolonas, aminoglucósidos, lincosamidas, péptidos y tetraciclinas, ampliamente distribuidos en los MAGs obtenidos. En este estudio se identificó la presencia de 11 especies Clostridiales en cuerpos de agua, las cuales transportan factores de virulencia y marcadores de resistencia, revelando un riesgo microbiológico en el río Pasto.
dc.description.abstractThe Clostridia class comprises bacteria with diverse roles, including commensal species and opportunistic pathogens. These bacteria are classified as anaerobic and are present in the gut of animals, including humans. Their spores are highly oxygen resistant, allowing them to expand their distribution in environmental samples such as soil and water. The presence of these bacteria in water bodies is an indicator of low quality, and areas of frequent contact between humans and water bodies are prone to multiple sources of contamination, including microorganisms. In particular, the presence of Clostridia in water is clinically relevant due to its pathogenic potential. This study aimed to detect Clostridial bacteria from water samples collected in the Pasto River between September 2022 and March 2023 and characterize them at the genomic level. We assembled Metagenome-Assembled Genomes (MAGs) in which we identified antimicrobial resistance markers and virulence factors, making this a relevant public health study. A total of 33 high-quality and four medium-quality MAGs were assembled, corresponding to 11 species of these bacteria, including Clostridium perfringens, Clostridium tetani, Clostridium botulinum, and Clostridioides difficile, which are medically relevant clostridial species. Notably, we detected the presence of the rarely reported species Pymaiobacter missiliensis and Clostridium sulfidigenes, both recently described Clostridial species that are poorly studied and for which there are no reported infections. Among the virulence factors identified were nag and related genes frequently found in strain 13 of C. perfringens. We also detected antibiotic-resistance markers associated with fluoroquinolones, aminoglycosides, lincosamides, peptides, and tetracyclines in the MAGs assembled. This study identified 11 Clostridial species in water bodies, which carry virulence factors and resistance markers, revealing a microbiological risk in the Pasto River.
dc.description.sponsorshipFacultad de Ciencias Naturales de la Universidad del Rosario
dc.description.sponsorshipSistema General de Regalías
dc.format.extent34 pp
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.48713/10336_43372
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/43372
dc.language.isospa
dc.publisherUniversidad del Rosario
dc.publisher.departmentFacultad de Ciencias Naturales
dc.publisher.programBiología
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
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dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectClostridiales
dc.subjectPatógenos
dc.subjectMarcadores de resistencia microbiana
dc.subjectFactores de Virulencia
dc.subject.keywordClostridials
dc.subject.keywordPathogens
dc.subject.keywordAntimicrobial Resistance Markers
dc.subject.keywordVirulence Factors
dc.titleCaracterización genómica de especies de Clostridiales Circulantes en el río Pasto
dc.title.TranslatedTitleGenomic Characterization of Circulating Clostridial Species in the Pasto River
dc.typebachelorThesis
dc.type.documentTrabajo de grado
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaTrabajo de grado
local.department.reportFacultad de Ciencias Naturales
local.regionesBogotá
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