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Mce4F Mycobacterium tuberculosis protein peptides can inhibit invasion of human cell lines

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dc.creatorRodríguez, Deisy Carolinaspa
dc.creatorOcampo, Marisolspa
dc.creatorVarela, Yahsonspa
dc.creatorCurtidor, Hernandospa
dc.creatorPatarroyo, Manuel A.
dc.creatorPatarroyo, Manuel Elkinspa
dc.date.accessioned2020-05-25T23:57:53Z
dc.date.available2020-05-25T23:57:53Z
dc.date.created2015spa
dc.description.abstractThis work was aimed at studying the Mycobacterium tuberculosis H37Rv Rv3494c protein, taking into account that it belongs to the mammalian cell entry family (mce) which is thought to have important functions in the disease's pathogenesis. The protein was characterized in silico; its presence on mycobacterial surface was confirmed by immunoelectron microscopy. High-activity binding peptides (HABPs) were identified by binding assays with 125I; their ability to inhibit mycobacterial entry to two cell lines (U937 alveolar macrophages and A549 epithelial cells) was ascertained and their role in bacterial entry was confirmed by fluorescent microsphere internalization assay. This protein's predicted alpha-helix structure was confirmed by circular dichroism of its peptides. All HABPs inhibited mycobacterial entry to cells and that the 38379 peptide (201IDQAGPFLQAQIRAGGDIKSY220) had high binding ability and inhibited the mycobacterial entry to both cell lines assayed here. Rv3494c peptides 38370 (21LSVMAIFYLRLPATFGIGTY40), 38373 (81HMRLNSGTAIPSNVTATVRSY100) and 38379 (201IDQAGPFLQAQIRAGGDIKSY220) showed to be HABP and inhibited mycobacterial entry to A549 cells and peptide 38382 (261RPSFPALAASLANLGRVGVIY280) bind to U937 and inhibited the mycobacterial entry to this cell line; all of these sequences play an important role in cell line recognition and invasion, and may thus be considered in the search for prophylactic candidates against tuberculosis. © FEMS 2015.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1093/femspd/ftu020
dc.identifier.issn2049632X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22763
dc.language.isoengspa
dc.publisherOxford University Pressspa
dc.relation.citationIssueNo. 3
dc.relation.citationTitlePathogens and Disease
dc.relation.citationVolumeVol. 73
dc.relation.ispartofPathogens and Disease, ISSN:2049632X, Vol.73, No.3 (2015)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84946762612&doi=10.1093%2ffemspd%2fftu020&partnerID=40&md5=334d2f8a88a7167484c835d7227d69f8spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordBacterial proteinspa
dc.subject.keywordbacterialeng
dc.subject.keywordIodine 125spa
dc.subject.keywordMce4f proteinspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAntiinfective agentspa
dc.subject.keywordBacterial antigenspa
dc.subject.keywordBiological productspa
dc.subject.keywordMembrane proteinspa
dc.subject.keywordMycobacterium tuberculosis antigensspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordA-549 cell linespa
dc.subject.keywordAlpha helixspa
dc.subject.keywordArticlespa
dc.subject.keywordBacterial virulencespa
dc.subject.keywordBeta sheetspa
dc.subject.keywordCell invasionspa
dc.subject.keywordCircular dichroismspa
dc.subject.keywordControlled studyspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordImmunoelectron microscopyspa
dc.subject.keywordInternalizationspa
dc.subject.keywordLung alveolus macrophagespa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPriority journalspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordProtein expressionspa
dc.subject.keywordU-937 cell linespa
dc.subject.keywordCell linespa
dc.subject.keywordDrug effectsspa
dc.subject.keywordEndocytosisspa
dc.subject.keywordEpithelium cellspa
dc.subject.keywordMacrophagespa
dc.subject.keywordMetabolismspa
dc.subject.keywordMicrobiologyspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordPhysiologyspa
dc.subject.keywordProtein conformationspa
dc.subject.keywordAnti-bacterial agentsspa
dc.subject.keywordAntigenseng
dc.subject.keywordBiological productsspa
dc.subject.keywordCell linespa
dc.subject.keywordCircular dichroismspa
dc.subject.keywordEndocytosisspa
dc.subject.keywordEpithelial cellsspa
dc.subject.keywordHumansspa
dc.subject.keywordMacrophagesspa
dc.subject.keywordMembrane proteinsspa
dc.subject.keywordMicroscopyeng
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordProtein conformationspa
dc.subject.keywordHabpspa
dc.subject.keywordInhibition and invasion assayspa
dc.subject.keywordMycobacterium tuberculosis h37rvspa
dc.subject.keywordRv3494c proteinspa
dc.subject.keywordTuberculosisspa
dc.titleMce4F Mycobacterium tuberculosis protein peptides can inhibit invasion of human cell linesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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