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dc.creatorUrbina, Adriana 
dc.creatorPalomino, Fernando 
dc.date.accessioned2019-10-01T15:22:01Z
dc.date.available2019-10-01T15:22:01Z
dc.date.created2018
dc.date.issued2018
dc.identifier.issn2531-1379
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/20373
dc.descriptionBackground: Reticulocytes are immature red blood cells containing RNA remnants. Their population kinetics has been documented under various in vivo and in vitro conditions, including after storage of red blood cells in blood banks. The purpose of this study was to describe the influence of blood bank storage on the kinetics of reticulocyte disappearance by in vitro culturing. Method: Samples of reticulocyte-enriched fractions (Percoll density-gradient) were obtained over different storage times from six red blood cell units stored in additive solution-1 (AS-1). Reticulocyte fractions were then cultured in enriched media at 37 °C and analyzed by flow cytometry with thiazole orange taking into account hemolysis. Results: Density-gradient enriched reticulocyte fractions were obtain from standard red blood cell units with <1% of reticulocytes. An exponential drop of reticulocytes was observed in cultures. The time taken for reticulocyte disappearance in cultures was shorter with increased blood bank storage time (144 ± 46 h at 0.5 weeks of storage and 15 ± 14 h in the sixth week). High fluorescence reticulocytes disappeared completely in 42.5 ± 8.5 h, medium fluorescence reticulocytes in 73.4 ± 20.8 h and low fluorescence reticulocytes in 269.9 ± 98.8 h in red blood cell units stored for half a week. These times significantly decreased in red blood cell units stored for more time. Conclusion: In vitro reticulocyte disappearance was significantly faster after prolonged storage of red blood cell units at 4 °C. The in vitro half-life at 0.5 weeks of storage was not significantly different from the values reported for fresh venous blood, but after the sixth week of storage, the half-lives were shorter. The possible explanation is that blood bank storage does not cause irreversible damage to the human reticulocyte maturational machinery. © 2018 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofHematology, Transfusion and Cell Therapy, ISSN:2531-1379, Vol. 40 (2018) pp. 143-150
dc.relation.urihttp://www.scielo.br/pdf/htct/v40n2/2531-1379-htct-40-02-0143.pdf
dc.subjectBovine Serum Albumin
dc.subjectBuffer
dc.subjectGlucose
dc.subjectHemoglobin
dc.subjectThiazole Orange
dc.subjectAlgorithm
dc.subjectAqueous Solution
dc.subjectArticle
dc.subjectBlood Bank
dc.subjectCell Culture
dc.subjectCell Fractionation
dc.subjectCell Isolation
dc.subjectCell Kinetics
dc.subjectCell Maturation
dc.subjectCell Survival
dc.subjectControlled Study
dc.subjectDensity Gradient
dc.subjectErythrocyte Count
dc.subjectErythrocyte Preservation
dc.subjectFlow Cytometry
dc.subjectFluorescence Analysis
dc.subjectHalf Life Time
dc.subjectHemolysis
dc.subjectHuman
dc.subjectKinetic Parameters
dc.subjectLight Scattering
dc.subjectMathematical Analysis
dc.subjectNormal Human
dc.subjectReticulocyte
dc.subjectSpectrophotometry
dc.subjectAlbúmina de suero bovino
dc.subjectBuffer
dc.subjectGlucosa
dc.subject.ddcFisiología humana 
dc.subject.lembGlucosa
dc.subject.lembHemoglobina
dc.subject.lembReticulocitos
dc.titleIn vitro kinetics of reticulocyte subtypes : maturation after red blood cell storage in additive solution-1 (AS-1)
dc.typearticle
dc.subject.keywordReticulocyte maturation
dc.subject.keywordBlood bank
dc.subject.keywordRed-blood-cell unit
dc.subject.keywordHemoglobina
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.source.bibliographicCitationKoury, M.J., Koury, S.T., Kopsombut, P., Bondurant, M.C., In vitro maturation of nascent reticulocytes to erythrocytes (2005) Blood, 105 (5), pp. 2168-2174
dc.creator.googleUrbina, Adriana
dc.creator.googlePalomino, Fernando
dc.identifier.doi10.1016/j.htct.2017.12.002


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