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dc.creatorPatarroyo M.E. 
dc.creatorAlba M.P. 
dc.creatorCurtidor H. 
dc.date.accessioned2020-05-25T23:58:02Z
dc.date.available2020-05-25T23:58:02Z
dc.date.created2011
dc.identifier.issn1969781
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22790
dc.description.abstractThe sporozoite microneme proteins essential for cell traversal, SPECT-1 and SPECT-2, are considered attractive pre-erythrocytic immune targets due to the key role they play in crossing of the malaria parasite across the dermis and the liver sinusoidal wall, prior to invasion of hepatocytes. In this study, the sequences of SPECT-1 and SPECT-2 were mapped using 20 mer-long synthetic peptides to identify high-activity binding peptides (HABPs) to HeLa cells. 17 HABPs with enzyme sensitive bindings to HeLa cells were identified: 3 predominantly ?-helical in SPECT-1, and 10 ?-helical and 4 ?-turns/random coils in SPECT-2. Immunofluorescence assays (IFA) with antibodies raised in rabbits against chemically synthesized B-cell epitopes suggests the presence of these two proteins in the micronemes and in sporozoite membrane. 1H NMR studies showed that HABPs located in the membrane-attack complex/perforin (MACPF) domain of SPECT-2 share high similarity with the 3D structure of C8?. Altogether, the results highlight the potential of including HABPs from SPECT-1 and SPECT-2 as components of a fully effective multistage, multiepitopic, minimal subunit-based synthetic vaccine against Plasmodium falciparum malaria. © 2010 Elsevier Inc. All rights reserved.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofPeptides, ISSN:1969781, Vol.32, No.1 (2011); pp. 154-160
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-78650519896&doi=10.1016%2fj.peptides.2010.09.026&partnerID=40&md5=8477715a4d060f467b9811b71843494d
dc.titleBiological and structural characteristics of the binding peptides from the sporozoite proteins essential for cell traversal (SPECT)-1 and -2
dc.typearticle
dc.subject.keywordAntibody
dc.subject.keywordCircumsporozoite protein
dc.subject.keywordComplement component c8
dc.subject.keywordPerforin
dc.subject.keywordSporozoite proteins essential for cell traversal 1
dc.subject.keywordSporozoite proteins essential for cell traversal 2
dc.subject.keywordUnclassified drug
dc.subject.keywordAlpha helix
dc.subject.keywordArticle
dc.subject.keywordEpitope mapping
dc.subject.keywordImmunofluorescence
dc.subject.keywordMolecular interaction
dc.subject.keywordPlasmodium falciparum
dc.subject.keywordPriority journal
dc.subject.keywordProtein binding
dc.subject.keywordProtein domain
dc.subject.keywordProtein structure
dc.subject.keywordProton nuclear magnetic resonance
dc.subject.keywordAmino acid sequence
dc.subject.keywordAnimals
dc.subject.keywordBinding sites
dc.subject.keywordHela cells
dc.subject.keywordHepatocytes
dc.subject.keywordHumans
dc.subject.keywordMolecular sequence data
dc.subject.keywordPeptides
dc.subject.keywordPlasmodium falciparum
dc.subject.keywordProtozoan proteins
dc.subject.keywordRabbits
dc.subject.keywordSporozoites
dc.subject.keywordOryctolagus cuniculus
dc.subject.keywordPlasmodium falciparum
dc.subject.keyword1h nmr
dc.subject.keywordAntimalarial vaccine
dc.subject.keywordHigh-activity binding peptides (habps)
dc.subject.keywordPlasmodium falciparum
dc.subject.keywordSpect-1
dc.subject.keywordSpect-2
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doihttps://doi.org/10.1016/j.peptides.2010.09.026
dc.relation.citationEndPage160
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage154
dc.relation.citationTitlePeptides
dc.relation.citationVolumeVol. 32
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR


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