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Identification of conserved erythrocyte binding regions in members of the Plasmodium falciparum Cys6 lipid raft-associated protein family

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García, Jeison
Curtidor, Hernando
Pinzón, Carlos G.
Vanegas, Magnolia
Moreno, Armando
Patarroyo, Manuel E.



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Detergent-resistant lipid raft membrane-associated Pf12, Pf38 and Pf41 proteins belong to the Cys6 family, whose members are implicated in Plasmodium falciparum invasion to erythrocytes. We have analyzed the interaction between 20-mer-long synthetic peptides spanning the entire Pf12, Pf38 and Pf41 sequences and erythrocytes. Eight high-activity binding peptides (HABPs) were identified in these proteins, which presented saturable bindings susceptible to erythrocytes' enzymatic treatment, and ?-turn, random coil and ?-helical elements as principal structural features. Some of these HABPs inhibited merozoite invasion in vitro, suggesting a possible role of Pf12, Pf38 and Pf41 during erythrocyte invasion and supporting their inclusion in the design of a fully effective antimalarial vaccine. © 2009 Elsevier Ltd. All rights reserved.
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Alanylglycyllysylvalylasparaginylasparaginyllysylvalyl cysteinyllysylisoleucylglutaminylglycyllysylprolylglycylglutamyll eucylvalylglycine , tertiary , Asparaginylthreonylthreonyllysylleucylasparaginylleucyl prolyllysylserylleucylasparaginylisoleucylprolylasparaginylaspartyl isoleucylleucylasparaginyltyrosinel , Cysteine derivative , Leucylhistidyllysylasparaginyllysylvalylthreonylaspartyll eucyllysylthreonylleucylisoleucylprolylglycyltyrosylalanylseryltyrosylthreonine , Malaria vaccine , Membrane protein , Methionylaspartylhistidyltyrosylasparaginylasparaginyl threonylphenylalanyltyrosylserylarginylleucylprolylserylleucyl isoleucylserylaspartylasparaginyltryptophan , Peptide derivative , Pf12 protein , Pf38 protein , Pf41 protein , Tyrosylserylisoleucyllysylprolylaspartylglycylcysteinylphenyl alanylserylasparaginylvalyltyrosylvalyllysylarginyltyrosylprolyl asparaginylglutamine , Unclassified drug , Valylisoleucylglycylserylserylmethionylphenylalanylmethionyl arginylarginylserylleucylthreonylprolylasparaginyllysylisoleucyl asparaginylglutamylvaline , Valylleucylarginylisoleucylhistidylisoleucylserylasparaginyl glycylvalylleucylarginyllysylisoleucylprolylglycylcysteinyl aspartylphenylalanylasparagine , Alpha helix , Article , Beta turn , Binding affinity , Binding site , Erythrocyte , Host parasite interaction , Human , Human cell , Lipid raft , Malaria , Merozoite , Plasmodium falciparum , Priority journal , Protein analysis , Protein function , Animals , Antigens , Erythrocytes , Humans , Plasmodium falciparum , Protein binding , Protein interaction mapping , Protein structure , Protein structure , Protozoan proteins , Receptors , Antimalarial vaccine , Cys6 family , High-activity binding peptides , Pf12 , Pf38 , Pf41 , Plasmodium falciparum