Comparative genomics identifies potential virulence factors in Clostridium tertium and C. paraputrificum
Ríos-Chaparro, Dora I
Díaz Arévalo, Diana
Patarroyo, Manuel A.
Lawley, Trevor D
Ramírez, Juan David
"Some well-known Clostridiales species such as Clostridium difficile and C. perfringens are agents of high impact diseases worldwide. Nevertheless, other foreseen Clostridiales species have recently emerged such as Clostridium tertium and C. paraputrificum. Three fecal isolates were identified as Clostridium tertium (Gcol.A2 and Gcol.A43) and C. paraputrificum (Gcol.A11) during public health screening for C. difficile infections in Colombia. C. paraputrificum genomes were highly diverse and contained large numbers of accessory genes. Genetic diversity and accessory gene percentage were lower among the C. tertium genomes than in the C. paraputrificum genomes. C. difficile tcdA and tcdB toxins encoding homologous sequences and other potential virulence factors were also identified. EndoA interferase, a toxic component of the type II toxin-antitoxin system, was found among the C. tertium genomes. toxA was the only toxin encoding gene detected in Gcol.A43, the Colombian isolate with an experimentally-determined high cytotoxic effect. Gcol.A2 and Gcol.A43 had higher sporulation efficiencies than Gcol.A11 (84.5%, 83.8% and 57.0%, respectively), as supported by the greater number of proteins associated with sporulation pathways in the C. tertium genomes compared with the C. paraputrificum genomes (33.3 and 28.4 on average, respectively). This work allowed complete genome description of two clostridiales species revealing high levels of intra-taxa diversity, accessory genomes containing virulence-factors encoding genes (especially in C. paraputrificum), with proteins involved in sporulation processes more highly represented in C. tertium. These finding suggest the need to advance in the study of those species with potential importance at public health level. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor and Francis Group."
Albendazole ; bacterial ; Bacterial toxin ; Clostridium difficile toxin a ; Clostridium difficile toxin b ; Endo a interferase ; Ertapenem ; Ivermectin ; Meropenem ; Metronidazole ; Rna 16s ; Teclozan ; Unclassified drug ; Virulence factor ; Bacterial protein ; Virulence factor ; Adult ; Amino acid sequence ; Antibiotic resistance ; Article ; Bacterial genome ; Bacterium culture ; Bacterium identification ; Bacterium isolation ; Biomass ; Cell viability ; Clostridium difficile infection ; Clostridium paraputrificum ; Clostridium tertium ; Comparative study ; Cryopreservation ; Cytotoxicity ; Dna extraction ; Feces analysis ; Gene ontology ; Gene sequence ; Genetic distance ; Genetic variability ; Genome size ; Genomics ; Health care facility ; Human ; Mass screening ; Matrix assisted laser desorption ionization time of flight mass spectrometry ; Metagenomics ; Nonhuman ; Optical density ; Peptoclostridium difficile ; Phylogeny ; Public health ; Sequence alignment ; Sporogenesis ; Taxonomy ; Toxin-antitoxin system ; Whole genome sequencing ; Clostridioides difficile ; Clostridium infection ; Clostridium tertium ; Colombia ; Comparative study ; Genetic variation ; Genetics ; Microbiology ; Bacterial proteins ; Clostridium difficile ; Clostridium infections ; Clostridium tertium ; Colombia ; Genetic variation ; Genome ; Genomics ; Humans ; Virulence factors ; Clostridial species ; Clostridium paraputrificum ; Clostridium tertium ; Genetic diversity ; Virulence factors ;
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