Ítem
Solo Metadatos
Sarconesiopsis magellanica (Diptera: Calliphoridae) excretions and secretions have potent antibacterial activity
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Autores
Díaz-Roa, Andrea
Gaona, María A.
Segura, Nydia A.
Suárez, Diana
Patarroyo, Manuel A.
Bello, Felio J.
Fecha
2014
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Editor
Elsevier
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Resumen
Abstract
The most important mechanism for combating infection using larval therapy depends on larval excretions and secretions (ES). The present work was aimed at evaluating Sarconesiopsis magellanica (Diptera: Calliphoridae) ES antibacterial activity in six bacterial strains (three Gram-positive and three Gram-negative) and comparing this to the effect of Lucilia sericata-derived ES. Antibacterial activity at 50. ?g/mL minimum inhibitory concentration (MIC) was observed for Staphylococcus epidermidis ATCC-12228 and Staphylococcus aureus ATCC-29213 strains, when the turbidimetry test involving S. magellanica ES was used; the rest of the bacterial strains (Staphylococcus aureus ATCC-6538, Pseudomonas aeruginosa ATCC-10145, Pseudomonas aeruginosa ATCC-9027 and Pseudomonas aeruginosa ATCC-27853) were inhibited at a 100. ?g/mL MIC. Twice the amount was required to inhibit the aforementioned bacteria with L. sericata-derived ES using this same technique; a similar trend was observed when the agar diffusion method was used instead. Furthermore, when the previously established MIC for each bacterial strain was used, their colonies became reduced following 1-6. h incubation with S. magellanica derived ES, whilst the reduction occurred from 2 to 6. hours with those from L. sericata. Although the MIC for each strain obtained with ciprofloxacin was lower than those established when using either blowfly derived-ES, the gradual reduction of the colonies occurred at a longer incubation time (6. h or more). The results showed that S. magellanica ES antibacterial activity was more potent and effective, compared to that of L. sericata-derived ES. © 2014 Elsevier B.V.
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Keywords
Natural product , Antiinfective agent , Insect protein , Antimicrobial activity , Comparative study , Excretion , Incubation , Inhibitor , Insect , Secretion , Turbidity , Agar diffusion , Antibacterial activity , Article , Bacterial strain , Biological activity , Bodily secretions , Calliphoridae , Colony formation , Controlled study , Drug potency , Excretion , Gram negative bacterium , Gram positive bacterium , In vitro study , Incubation time , Larval stage , Lucilia sericata , Minimum inhibitory concentration , Nonhuman , Pseudomonas aeruginosa , Sarconesiopsis magellanica , Species comparison , Staphylococcus aureus , Staphylococcus epidermidis , Animal , Bodily secretions , Chemistry , Diptera , Drug effects , Metabolism , Microbial sensitivity test , Staphylococcus , Animals , Anti-bacterial agents , Bodily secretions , Diptera , Insect proteins , Microbial sensitivity tests , Pseudomonas aeruginosa , Staphylococcus , Antibacterial activity , Excretions and secretions , Larval therapy , Minimum inhibitory concentration , Sarconesiopsis magellanica
