A TGF-? mediated regulatory mechanism modulates the T cell immune response to rotavirus in adults but not in children
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Mesa, Martha C.
Franco, Manuel A.
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Children with acute RV-gastroenteritis (GE) had low or undetectable levels of circulating IFN-?+, IL-13+, IL-2+, IL-10+ or IL-17+ RV-T cells. IFN-?+ T cells and low frequencies of IL-10+ and IL-2+ CD4+ T cells were found in adults with RV-GE during acute and convalescence phases, respectively. Circulating single IFN-?+ > double IFN-?+/IL-2+ > single IL-2+RV-CD4+T cells were observed in healthy adults. In this group, frequencies of IFN-?+ RV-T cells increased after removing CD25+cells, blocking TGF-? with its natural inhibitor, LAP, or inhibiting TGF-?RI signalling pathway with ALK5i. The frequencies of IFN-?+ RV-T cells were also incremented in PBMC depleted of CD25+cells and treated with ALK5i, suggesting that TGF? inhibition may be independent of Treg cells. The ALK5i effect was observed in adults but not in children with RV-GE, who had normal numbers of TGF-?+ Treg cells. Thus, a TGF-?-mediated regulatory mechanism that modulates RV-T cells in adults is not evident in children.
Rotavirus , Humans , Interferon-gamma , Interleukin 13 , Interleukin-2 , Interleukin-10 , Interleukin-17 , Transforming growth factor betaT-lymphocytes , T-lymphocytes , regulatory