Application of Real-Time PCR Assays for the Diagnosis of Histoplasmosis in Human FFPE Tissues Using Three Molecular Targets

Caceres Contreras, Diego Hernando

doi:http://doi.org/10.3390/jof9070700

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Application of Real-Time PCR Assays for the Diagnosis of Histoplasmosis in Human FFPE Tissues Using Three Molecular Targets

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dc.creator	Caceres Contreras, Diego Hernando	spa
dc.date.accessioned	2024-01-31T18:20:04Z
dc.date.available	2024-01-31T18:20:04Z
dc.date.created	2023-07-01	spa
dc.date.issued	2023	spa
dc.description	Histoplasmosis is a fungal infection caused by the thermally dimorphic fungus Histoplasma capsulatum. This infection causes significant morbidity and mortality in people living with HIV/AIDS, especially in countries with limited resources. Currently used diagnostic tests rely on culture and serology but with some limitations. No molecular assays are commercially available and the results from different reports have been variable. We aimed to evaluate quantitative real-time PCR (qPCR) targeting three protein-coding genes of Histoplasma capsulatum (100-kDa, H and M antigens) for detection of this fungus in formalin-fixed paraffin-embedded (FFPE) samples from patients with proven histoplasmosis. The sensitivity of 100-kDa, H and M qPCR assays were 93.9%, 91% and 57%, respectively. The specificity of 100-kDa qPCR was 93% when compared against samples from patients with other mycoses and other infections, and 100% when samples from patients with non-infectious diseases were used as controls. Our findings demonstrate that real-time PCR assays targeting 100-kDa and H antigen showed the most reliable results and can be successfully used for diagnosing this mycosis when testing FFPE samples.	spa
dc.format.mimetype	application/pdf	spa
dc.identifier.doi	http://doi.org/10.3390/jof9070700	spa
dc.identifier.issn	2309-608X	spa
dc.identifier.uri	https://repository.urosario.edu.co/handle/10336/42093
dc.language.iso	eng	spa
dc.publisher	Universidad del Rosario	spa
dc.relation.uri	https://www.mdpi.com/2309-608X/9/7/700	spa
dc.rights	Attribution-NonCommercial-ShareAlike 4.0 International	spa
dc.rights.accesRights	info:eu-repo/semantics/openAccess	spa
dc.rights.acceso	Abierto (Texto Completo)	spa
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	spa
dc.source	Journal of Fungi	spa
dc.source.instname	instname:Universidad del Rosario	spa
dc.source.reponame	reponame:Repositorio Institucional EdocUR	spa
dc.subject	Histoplasmosis	spa
dc.subject	Histoplasma capsulatum	spa
dc.subject	Diagnosis	spa
dc.subject	Real time PCR	spa
dc.subject	FFPE tissues	spa
dc.title	Application of Real-Time PCR Assays for the Diagnosis of Histoplasmosis in Human FFPE Tissues Using Three Molecular Targets	spa
dc.type	article	spa
dc.type.hasVersion	info:eu-repo/semantics/publishedVersion	spa
dc.type.spa	Artículo	spa