Ítem
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Characterizing T?cell receptor ??variable gene in Aotus nancymaae owl monkey peripheral blood
Título de la revista
Autores
Guerrero, J.E.
Pacheco, D. P.
Suárez, C. F.
Martínez, P.
Aristizabal, F.
Moncada, C. A.
Patarroyo, M. E.
Patarroyo, M. A.
Fecha
2003-11-11
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John Wiley & Sons
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Resumen
Abstract
Gd T lymphocytes have a heterodimeric complex formed by theassociation of g and d chains as receptor. Proliferation of this lymphocytepopulation has been observed, when infection by several pathogens such asMycobacterium tuberculosis and Plasmodium spp. occurs. The New WorldMonkey Aotus nancymaae has become a very good experimental model forthe immunological and physiopathological study of these infectious agents.The A. nancymaae g-variable region was characterized from peripheral bloodsamples by using cDNA and genomic DNA polymerase chain reactionamplification, DNA sequencing, and dot-blot hybridization techniques.Seventeen different T-cell receptor g-variable (TCRGV) sequences wereobtained. These sequences were distributed among TCRGV subsets 1, 2, or 3,according to human subset classification. Although no subset 4 amplificationwas obtained, this subset was detected by dot-blot hybridization. Thepresence of these 4 subsets resembles the behavior displayed by ‘gd-lowspecies’ (humans and mice), where high diversity among these lymphocytescan be observed. Homologies greater than 70% were found with respect tohumans. Sequence convergence between human and A. nancymaae subsets1 and 3 highlights Aotus as a promising model for studying these lymphocytefunctions.
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Keywords
Aotus , Evolution , Gd T lymphocytes , Platyrrhini , T-cell recepto
