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Biological characterisation of yellow fever 17D strain plaque variants

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dc.creatorRojas M.O.spa
dc.creatorCamacho M.spa
dc.creatorGrosso M.V.spa
dc.date.accessioned2020-05-25T23:59:32Z
dc.date.available2020-05-25T23:59:32Z
dc.date.created2009spa
dc.description.abstractThe Colombian 17D vaccine contains at least four phenotypes denominated small (0.3 - 1.2 mm), medium (1.3 - 2.1 mm) large (2.2 - 3.0 mm) and extra-large ( and gt;3.1 mm). These phenotypes composition and percentage distribution vary between different production lots and between ampoules from a particular lot. Each variant was cloned by diluting the vaccine and its virulent effect was analysed in young mice. The small plaque phenotype was slightly under represented in the lots analysed and showed similar virulence to the neurotropic French wild strain (LD50 and gt;10-6), whilst the medium- sized phe-notype predominated and was the most attenuated (LD50:10-4). The large and extra-large phenotypes showed intermediate virulence (LD50:10-5) regarding the others. Sequence analysis of variants within the region between the 3'NS5 end and the beginning of 3'NCR showed the closeness between those variants having some degree of virulence and between the attenuated variant and the Colombian vaccine. The 17D vaccine's heterogeneity constitutes evidence of RNA virus quasi-specie structure and shows how adverse yellow fever vaccine reaction can be associated with applying vaccines produced from attenuated viral strains.eng
dc.format.mimetypeapplication/pdf
dc.identifier.issn1202804
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23061
dc.language.isoengspa
dc.relation.citationEndPage24
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage7
dc.relation.citationTitleRevista Colombiana de Quimica
dc.relation.citationVolumeVol. 38
dc.relation.ispartofRevista Colombiana de Quimica, ISSN:1202804, Vol.38, No.1 (2009); pp. 7-24spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-75849162287&partnerID=40&md5=f807ea42369ef9b561318737c015ed8aspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keyword17D vaccinespa
dc.subject.keywordAdverse vaccine eventsspa
dc.subject.keywordFlavivirusspa
dc.subject.keywordYellow fever virusspa
dc.titleBiological characterisation of yellow fever 17D strain plaque variantsspa
dc.title.TranslatedTitleCaracterización biológica de variantes de placa de la cepa vacunal 17D contra la fiebre amarillaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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