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Metagenomic Analysis of Surface Waters and Wastewater in the Colombian Andean Highlands: Implications for Health and Disease

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dc.creatorMuñoz Díaz, Claudia Marinaspa
dc.creatorRamírez, Juan Davidspa
dc.creatorGaleano, Luis‑Alejandrospa
dc.creatorHidalgo‑Troya, Arseniospa
dc.creatorBallesteros, Nathaliaspa
dc.creatorVega, Lauraspa
dc.creatorPatiño, Luz H.spa
dc.creatorCamargo, Milenaspa
dc.creatorVelásquez‑Ortiz, Nataliaspa
dc.creatorPáez‑Triana, Luisaspa
dc.creatorUrrea, Vanessaspa
dc.date.accessioned2025-07-21T16:49:32Z
dc.date.available2025-07-21T16:49:32Z
dc.date.created2025-04-01spa
dc.date.issued2025-04-01spa
dc.description.abstractUrban water bodies serve as critical reservoirs of microbial diversity, with major implications for public health and environmental quality. This study aimed to characterize the microbial diversity of surface waters and wastewater from the Pasto River in the Colombian Andean Highlands, offering insights that may support water quality monitoring efforts. Sampling was conducted at three river sites and one wastewater location. Standard physicochemical and microbiological analyses were performed, including real-time PCR to detect protozoan pathogens Giardia spp. and Cryptosporidium spp. Metagenomic sequencing provided an in-depth taxonomic and functional profile of microbial communities through two complementary approaches: (i) read-based analysis to identify abundant families and species, both pathogenic and beneficial, and (ii) detection of health-related molecular markers, including antimicrobial resistance markers and virulence factors. Physicochemical analyses showed distinct profiles between wastewater and surface water, with wastewater exhibiting elevated levels of suspended solids (113.6 mg/L), biochemical oxygen demand (BOD, 311.2 mg/L), and chemical oxygen demand (COD, 426.7 mg/L). Real-time PCR detected Giardia spp. DNA in 75% (76/102) of the samples and Cryptosporidium spp. DNA in 94% (96/102) of samples. The metagenomic read-based profiling identified Aeromonas media as a prevalent pathogen and Polaromonas naphthalenivorans as a potential biodegradative agent. The metagenomic assembly produced 270 high-quality genomes, revealing 16 bacterial species (e. g., Acinetobacter johnsonii and Megamonas funiformis) that provided insights into fecal contaminants and native aquatic microbes. Functional profiling further revealed a high prevalence of antimicrobial resistance markers, particularly for tetracyclines, aminoglycosides, and macrolides, with the highest abundance found in wastewater samples. Additionally, virulence factors were notably present in Zoogloea ramigera. The findings underscore the value of metagenomic profiling as a comprehensive tool for water quality monitoring, facilitating the detection of pathogens, beneficial species, and molecular markers indicative of potential health risks. This approach supports continuous monitoring efforts, offering actionable data for water management strategies to safeguard public health and maintain ecological integrity.eng
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1007/s00284-024-04019-7spa
dc.identifier.issn0343-8651spa
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/46088
dc.language.isoengspa
dc.publisherSpringer Nature Linkspa
dc.relation.ispartofCurrent Microbiology (2025) 82:162spa
dc.relation.urihttps://link.springer.com/article/10.1007/s00284-024-04019-7spa
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourceCurrent Microbiologyspa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordUrban water bodieseng
dc.subject.keywordMicrobial diversityeng
dc.subject.keywordPublic healtheng
dc.subject.keywordEnvironmental qualityeng
dc.subject.keywordPasto Rivereng
dc.subject.keywordColombian Andean Highlandseng
dc.subject.keywordWater quality monitoringeng
dc.subject.keywordSurface waterseng
dc.subject.keywordWastewatereng
dc.subject.keywordPhysicochemical analysiseng
dc.subject.keywordMicrobiological analysiseng
dc.subject.keywordReal-time PCReng
dc.subject.keywordProtozoan pathogenseng
dc.subject.keywordGiardia spp.eng
dc.subject.keywordCryptosporidium spp.eng
dc.subject.keywordMetagenomic sequencingeng
dc.subject.keywordTaxonomic profilingeng
dc.subject.keywordFunctional profilingeng
dc.subject.keywordRead-based analysiseng
dc.subject.keywordAbundant familieseng
dc.subject.keywordAbundant specieseng
dc.subject.keywordPathogenic specieseng
dc.subject.keywordBeneficial specieseng
dc.subject.keywordHealth-related molecular markerseng
dc.subject.keywordAntimicrobial resistance markerseng
dc.subject.keywordVirulence factorseng
dc.subject.keywordSuspended solidseng
dc.subject.keywordBiochemical oxygen demand (BOD)eng
dc.subject.keywordChemical oxygen demand (COD)eng
dc.subject.keywordAeromonas mediaeng
dc.subject.keywordPolaromonas naphthalenivoranseng
dc.subject.keywordMetagenomic assemblyeng
dc.subject.keywordHigh-quality genomeseng
dc.subject.keywordBacterial specieseng
dc.subject.keywordAcinetobacter johnsoniieng
dc.subject.keywordMegamonas funiformiseng
dc.subject.keywordFecal contaminantseng
dc.subject.keywordNative aquatic microbeseng
dc.subject.keywordTetracyclineseng
dc.subject.keywordAminoglycosideseng
dc.subject.keywordMacrolideseng
dc.subject.keywordZoogloea ramigeraeng
dc.subject.keywordEcological integrityeng
dc.titleMetagenomic Analysis of Surface Waters and Wastewater in the Colombian Andean Highlands: Implications for Health and Diseasespa
dc.typearticlespa
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.type.spaArtículo de Investigaciónspa
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