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Conserved high activity binding peptides from the Plasmodium falciparum Pf34 rhoptry protein inhibit merozoites in vitro invasion of red blood cells

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dc.creatorArévalo-Pinzón, Gabrielaspa
dc.creatorCurtidor, Hernandospa
dc.creatorVanegas, Magnoliaspa
dc.creatorVizcaíno, Carolinaspa
dc.creatorPatarroyo, Manuel A.spa
dc.creatorPatarroyo, Manuel E.spa
dc.date.accessioned2020-05-25T23:58:00Z
dc.date.available2020-05-25T23:58:00Z
dc.date.created2010spa
dc.description.abstractRhoptries are specialized secretory organelles found in all members of the genus Plasmodium whose proteins have been considered as promising vaccine candidates due to their involvement in cell invasion and the formation of the parasitophorous vacuole (PV). The Plasmodium falciparum Pf34 protein was recently identified as a rhoptry-neck protein located in detergent-resistant microdomains (DRMs) that is expressed in mature intraerythrocytic parasite stages, but its biological function is still unknown. Receptor-ligand assays carried out in this study found that peptides 36,051 ( 101DKKFSESLKAHMDHLKILNN120Y), 36,053 ( 141KKYIIKEIQNNKYLNKEKKS160), 36,055 ( 181WLESVNNIEEKSNILKNIKS200Y) and 36,056 ( 201QLLNNIASLNHTLSEEIKNI220Y), located in the central portion of Pf34, were found to establish protease-sensitive interactions of high affinity and specificity with receptors on the surface of red blood cell (RBCs). In vitro assays showed that Pf34 high activity binding peptides (HABPs) inhibit invasion of RBCs by P. falciparum merozoites, therefore suggesting that Pf34 could act as an adhesin during invasion and supporting the inclusion of Pf34 HABPs in further studies to develop antimalarial control methods. © 2010 Elsevier Inc. All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.peptides.2010.07.009
dc.identifier.issn1969781
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22785
dc.language.isoengspa
dc.relation.citationEndPage1994
dc.relation.citationIssueNo. 11
dc.relation.citationStartPage1987
dc.relation.citationTitlePeptides
dc.relation.citationVolumeVol. 31
dc.relation.ispartofPeptides, ISSN:1969781, Vol.31, No.11 (2010); pp. 1987-1994spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-77957856509&doi=10.1016%2fj.peptides.2010.07.009&partnerID=40&md5=b24e424397e96b8458f717bef97eaa02spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAdhesinspa
dc.subject.keywordAntimalarial agentspa
dc.subject.keywordHigh activity binding peptide 36051spa
dc.subject.keywordHigh activity binding peptide 36053spa
dc.subject.keywordHigh activity binding peptide 36055spa
dc.subject.keywordHigh activity binding peptide 36056spa
dc.subject.keywordPeptide derivativespa
dc.subject.keywordPlasmodium falciparum pf34 proteinspa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordArticlespa
dc.subject.keywordCell invasionspa
dc.subject.keywordCircular dichroismspa
dc.subject.keywordDrug inhibitionspa
dc.subject.keywordDrug receptor bindingspa
dc.subject.keywordErythrocytespa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordIn vitro studyspa
dc.subject.keywordMerozoitespa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPriority journalspa
dc.subject.keywordProtein interactionspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimalsspa
dc.subject.keywordErythrocytesspa
dc.subject.keywordHumansspa
dc.subject.keywordMembrane glycoproteinsspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordDetergent-resistant microdomainsspa
dc.subject.keywordGpi-anchoredspa
dc.subject.keywordPeptidesspa
dc.subject.keywordReceptorsspa
dc.subject.keywordVaccinespa
dc.titleConserved high activity binding peptides from the Plasmodium falciparum Pf34 rhoptry protein inhibit merozoites in vitro invasion of red blood cellsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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