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Peptides from the Mycobacterium tuberculosis Rv1980c protein involved in human cell infection: Insights into new synthetic subunit vaccine candidates

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dc.creatorRodríguez Burbano, Diana Consuelo
dc.creatorVizcaíno, Carolinaspa
dc.creatorOcampo, Marisol
dc.creatorCurtidor, Hernandospa
dc.creatorPinto, Martaspa
dc.creatorPatarroyo, Manuel Elkinspa
dc.creatorPatarroyo, Manuel A.
dc.date.accessioned2020-05-26T00:02:46Z
dc.date.available2020-05-26T00:02:46Z
dc.date.created2010spa
dc.description.abstractMycobacterium tuberculosis infection continues to be a major cause of morbidity and mortality throughout the world. The vast complexity of the intracellular pathogen M. tuberculosis and the diverse mechanisms by which it can invade host cells highlight the importance of developing a fully protective vaccine. Our vaccine development strategy consists of including fragments from multiple mycobacterial proteins involved in cell invasion. The aim of this study was to identify high activity binding peptides (HABPs) in the immunogenic protein Rv1980c from M. tuberculosis H37Rv with the ability to inhibit mycobacterial invasion into U937 monocyte-derived macrophages and A549 cells. The presence and transcription of the Rv1980c gene was assessed in members belonging to the M. tuberculosis complex and other nontuberculous mycobacteria by PCR and RT-PCR, respectively. Cell surface localization was confirmed by immunoelectron microscopy. Three peptides binding with high activity to U937 cells and one to A549 cells were identified. HABPs 31100, 31101, and 31107 inhibited invasion of M. tuberculosis into A549 and U937 cells and therefore could be promising candidates for the design of a subunit-based antituberculous vaccine. © 2010 by Walter de Gruyter Berlin New York.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1515/BC.2010.019
dc.identifier.issn14316730
dc.identifier.issn14374315
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23522
dc.language.isoengspa
dc.relation.citationEndPage217
dc.relation.citationIssueNo. 43892
dc.relation.citationStartPage207
dc.relation.citationTitleBiological Chemistry
dc.relation.citationVolumeVol. 391
dc.relation.ispartofBiological Chemistry, ISSN:14316730, 14374315, Vol.391, No.43892 (2010); pp. 207-217spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-77749341488&doi=10.1515%2fBC.2010.019&partnerID=40&md5=bf7333e6188d1e8e692e93f5d7780cf9spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordBacterial proteinspa
dc.subject.keywordBinding proteinspa
dc.subject.keywordHigh activity binding peptide 31100spa
dc.subject.keywordHigh activity binding peptide 31101spa
dc.subject.keywordHigh activity binding peptide 31107spa
dc.subject.keywordProtein rv1980cspa
dc.subject.keywordSubunit vaccinespa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordArticlespa
dc.subject.keywordBacterial genespa
dc.subject.keywordBinding affinityspa
dc.subject.keywordCell invasionspa
dc.subject.keywordCell surfacespa
dc.subject.keywordControlled studyspa
dc.subject.keywordGenetic transcriptionspa
dc.subject.keywordHost cellspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordImmunoelectron microscopyspa
dc.subject.keywordMacrophagespa
dc.subject.keywordMonocytespa
dc.subject.keywordMorbidityspa
dc.subject.keywordMortalityspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPriority journalspa
dc.subject.keywordReverse transcription polymerase chain reactionspa
dc.subject.keywordRv1980c genespa
dc.subject.keywordTuberculosisspa
dc.subject.keywordBacterial proteinsspa
dc.subject.keywordBacterial vaccinesspa
dc.subject.keywordBinding sitesspa
dc.subject.keywordBlottingeng
dc.subject.keywordCellseng
dc.subject.keywordHumansspa
dc.subject.keywordModelseng
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordPeptide fragmentsspa
dc.subject.keywordPneumocytesspa
dc.subject.keywordTranscriptioneng
dc.subject.keywordVaccineseng
dc.subject.keywordCorynebacterineaespa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordBinding interactionsspa
dc.subject.keywordImmunodetectionspa
dc.subject.keywordInternalizationspa
dc.subject.keywordInvasion inhibitionspa
dc.subject.keywordPeptidespa
dc.subject.keywordTuberculosisspa
dc.titlePeptides from the Mycobacterium tuberculosis Rv1980c protein involved in human cell infection: Insights into new synthetic subunit vaccine candidatesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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