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Genomic analyses reveal moderate levels of ploidy, high heterozygosity and structural variations in a Colombian isolate of Leishmania (Leishmania) amazonensis

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dc.creatorPatino, Luz H.spa
dc.creatorMuskus, Carlosspa
dc.creatorMuñoz, Marinaspa
dc.creatorRamírez, Juan David
dc.date.accessioned2020-05-26T00:04:48Z
dc.date.available2020-05-26T00:04:48Z
dc.date.created2020spa
dc.description.abstractLeishmania amazonensis is one of the causative agents of the different forms of cutaneous leishmaniasis present in Latin America. This species has been isolated from humans and animals (canine/feline) in some endemic regions of Colombia. Therefore, L. amazonensis is of great relevance at the clinical and epidemiological levels in medicine and veterinary science. Until now, very few genomes from this species are available. Here, we report the complete genome sequence of a laboratory-adapted L. amazonensis strain isolated from a human patient with clinical manifestation of cutaneous leishmaniasis in Colombia. The genome sequence not only allowed inter and intra-species comparative analyses to be performed with the sequenced genomes of L. amazonensis strains from different geographical regions, but also increased our knowledge about the genomic behavior of this L. amazonensis Colombian strain. This isolate was also characterized in terms of single nucleotide polymorphisms, chromosome and gene copy number variations (CNVs). The results revealed moderate aneuploidy, CNVs in genes involved in the virulence, growth, and survival of the parasite, and in the distributions of the multicopy genes on some chromosomes, as well as a high level of heterozygosity. The data confirmed previous reports that identified unique variations in L. amazonensis, suggesting aneuploidy may not have a high fitness cost and may allow the rapid generation of diversity in Leishmania parasites growing normally. © 2019 Elsevier B.V.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.actatropica.2019.105296
dc.identifier.issn0001706X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23719
dc.language.isoengspa
dc.publisherElsevier B.V.spa
dc.relation.citationTitleActa Tropica
dc.relation.citationVolumeVol. 203
dc.relation.ispartofActa Tropica, ISSN:0001706X, Vol.203,(2020)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85077322210&doi=10.1016%2fj.actatropica.2019.105296&partnerID=40&md5=c8abe2a6e2e6ef111342546bf1ac7f20spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordEpidemiologyspa
dc.subject.keywordGenetic variationspa
dc.subject.keywordGenomicsspa
dc.subject.keywordHeterozygosityspa
dc.subject.keywordLeishmaniasisspa
dc.subject.keywordParasitespa
dc.subject.keywordPloidyspa
dc.subject.keywordAneuploidyspa
dc.subject.keywordArticlespa
dc.subject.keywordChromosome variantspa
dc.subject.keywordColombiaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCopy number variationspa
dc.subject.keywordGenetic variationspa
dc.subject.keywordGenome analysisspa
dc.subject.keywordHeterozygosityspa
dc.subject.keywordLeishmania amazonensisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordParasite developmentspa
dc.subject.keywordParasite survivalspa
dc.subject.keywordParasite virulencespa
dc.subject.keywordPhylogenyspa
dc.subject.keywordPloidyspa
dc.subject.keywordSingle nucleotide polymorphismspa
dc.subject.keywordSpecies comparisonspa
dc.subject.keywordWhole genome sequencingspa
dc.subject.keywordColombiaspa
dc.subject.keywordAnimaliaspa
dc.subject.keywordLeishmania amazonensisspa
dc.subject.keywordAneuploidyspa
dc.subject.keywordCopy number variationspa
dc.subject.keywordDiversityspa
dc.subject.keywordDna-seqspa
dc.subject.keywordGenome sequencingspa
dc.subject.keywordLspa
dc.subject.keywordAmazonensisspa
dc.subject.keywordParasitesspa
dc.titleGenomic analyses reveal moderate levels of ploidy, high heterozygosity and structural variations in a Colombian isolate of Leishmania (Leishmania) amazonensisspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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