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A novel approach for HLA-A typing in formalin-fixed paraffin-embedded-derived DNA

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dc.creatorVillabona, Lisaspa
dc.creatorLeon Rodriguez, Daniel Aspa
dc.creatorAndersson, Emilia Kspa
dc.creatorSeliger, Barbaraspa
dc.creatorDalianis, Tinaspa
dc.creatorMasucci, Giuseppe Vspa
dc.date.accessioned2020-05-25T23:57:05Z
dc.date.available2020-05-25T23:57:05Z
dc.date.created2014spa
dc.description.abstractThe aim of this study was to establish a novel approach for human leukocyte antigen (HLA)-typing from formalin-fixed paraffin-embedded-derived DNA. HLAs can be a prognostic factor in cancer and have an extensive polymorphism. This polymorphism is predominantly restricted to exons, which encode the peptide-binding domain of the protein. Formalin-fixed paraffin-embedded material is routinely collected in the clinic and therefore a great source of DNA for genetic analyses. However, its low quality due to fragmentation and nucleotide changes has often created obstacles in designing genetic assays. In this study, we amplified the most polymorphic exons of the HLA-A gene, exons 2, 3, and 4, in 16 formalin-fixed paraffin-embedded samples >10 years old. These tissue samples belonged to patients already HLA-typed by peripheral blood samples at the routine laboratory. Acquired amplification products were used for sequencing, which provided enough information to establish an HLA allele. The same method was applied to DNA extracted from peripheral blood from a healthy volunteer with known HLA type. Of the samples, 14/16 (88%) were successfully typed, in one sample only one of the alleles could be determined, and in one sample no allele could be determined. The amplification of the most polymorphic exons of HLA-A was a successful alternative when DNA quality prevented positive results with previously described methods. The method is usable when an HLA type is needed but the patients are deceased and/or no whole blood samples can be collected. It has thus potential to be used in several fields such as the clinic, research, and forensic science. © 2014 USCAP, Inc All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1038/modpathol.2013.210
dc.identifier.issn15300285
dc.identifier.issn08933952
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22601
dc.language.isoengspa
dc.publisherNature Publishing Groupspa
dc.relation.citationEndPage1305
dc.relation.citationIssueNo. 9
dc.relation.citationStartPage1296
dc.relation.citationTitleModern Pathology
dc.relation.citationVolumeVol. 27
dc.relation.ispartofModern Pathology, ISSN:15300285, 08933952, Vol.27, No.9 (2014); pp. 1296-1305spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84925229006&doi=10.1038%2fmodpathol.2013.210&partnerID=40&md5=3e182c447fad3c69282e9633a5019bc9spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordDnaspa
dc.subject.keywordFormaldehydespa
dc.subject.keywordHla a antigenspa
dc.subject.keywordParaffinspa
dc.subject.keywordDnaspa
dc.subject.keywordFixativespa
dc.subject.keywordFormaldehydespa
dc.subject.keywordHla a antigenspa
dc.subject.keywordParaffinspa
dc.subject.keywordPrimer dnaspa
dc.subject.keywordAllelespa
dc.subject.keywordArticlespa
dc.subject.keywordBlood samplingspa
dc.subject.keywordClinical articlespa
dc.subject.keywordControlled studyspa
dc.subject.keywordDna extractionspa
dc.subject.keywordDna fingerprintingspa
dc.subject.keywordDna polymorphismspa
dc.subject.keywordExonspa
dc.subject.keywordFemalespa
dc.subject.keywordGene amplificationspa
dc.subject.keywordGene sequencespa
dc.subject.keywordGenetic analysisspa
dc.subject.keywordHla typingspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman tissuespa
dc.subject.keywordOvary cancerspa
dc.subject.keywordPriority journalspa
dc.subject.keywordGeneticsspa
dc.subject.keywordHistocompatibility testspa
dc.subject.keywordMolecular geneticsspa
dc.subject.keywordNucleotide sequencespa
dc.subject.keywordOvary tumorspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordProceduresspa
dc.subject.keywordAllelesspa
dc.subject.keywordBase sequencespa
dc.subject.keywordDnaspa
dc.subject.keywordDna primersspa
dc.subject.keywordExonsspa
dc.subject.keywordFemalespa
dc.subject.keywordFixativesspa
dc.subject.keywordFormaldehydespa
dc.subject.keywordGene amplificationspa
dc.subject.keywordHistocompatibility testingspa
dc.subject.keywordHla-a antigensspa
dc.subject.keywordHumansspa
dc.subject.keywordMolecular sequence dataspa
dc.subject.keywordOvarian neoplasmsspa
dc.subject.keywordParaffin embeddingspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordFormalin-fixed paraffin-embedded tissuespa
dc.subject.keywordHla typingspa
dc.subject.keywordOvarian cancer sequencingspa
dc.titleA novel approach for HLA-A typing in formalin-fixed paraffin-embedded-derived DNAspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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