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Comparative study of Candida spp. isolates: Identification and echinocandin susceptibility in isolates obtained from blood cultures in 15 hospitals in Medellín, Colombia

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dc.creatorBerrio I.spa
dc.creatorMaldonado N.spa
dc.creatorDe Bedout C.spa
dc.creatorArango K.spa
dc.creatorCano L.E.spa
dc.creatorValencia Y.spa
dc.creatorJiménez-Ortigosa C.spa
dc.creatorPerlin D.S.spa
dc.creatorGómez B.L.spa
dc.creatorRobledo C.spa
dc.creatorRobledo J.spa
dc.creatorGrupo GERMENspa
dc.date.accessioned2020-05-25T23:56:12Z
dc.date.available2020-05-25T23:56:12Z
dc.date.created2018spa
dc.description.abstractObjectives: Invasive candidiasis has a high impact on morbidity and mortality in hospitalised patients. Accurate and timely methods for identification of Candida spp. and determination of echinocandin susceptibility have become a priority for clinical microbiology laboratories. Methods: This study was performed to compare matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) identification with sequencing of the D1/D2 region of the rRNA gene complex 28 subunit in 147 Candida spp. isolates obtained from patients with candidaemia. Antimicrobial susceptibility testing was performed by broth microdilution (BMD) and Etest. Sequencing of the FKS1 and FKS2 genes was performed. Results: The most common species isolated were Candida albicans (40.8%), followed by Candida parapsilosis (23.1%) and Candida tropicalis (17.0%). Overall agreement between the results of identification by MALDI-TOF/MS and molecular identification was 99.3%. Anidulafungin and caspofungin susceptibility by the BMD method was 98.0% and 88.4%, respectively. Susceptibility to anidulafungin and caspofungin by Etest was 93.9% and 98.6%, respectively. Categorical agreement between Etest and BMD was 91.8% for anidulafungin and 89.8% for caspofungin, with lower agreements in C. parapsilosis for anidulafungin (76.5%) and C. glabrata for caspofungin (40.0%). No mutations related to resistance were found in the FKS genes, although 54 isolates presented synonymous polymorphisms in the hotspots sequenced. Conclusions: MALDI-TOF/MS is a good alternative for routine identification of Candida spp. isolates. DNA sequencing of the FKS genes suggested that the isolates analysed were susceptible to echinocandins; alternatively, unknown resistance mechanisms or limitations related to antifungal susceptibility tests may explain the resistance found in a few isolates. © 2017 International Society for Chemotherapy of Infection and Cancereng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.jgar.2017.11.010
dc.identifier.issn22137165
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22357
dc.language.isoengspa
dc.publisherElsevier Ltdspa
dc.relation.citationEndPage260
dc.relation.citationStartPage254
dc.relation.citationTitleJournal of Global Antimicrobial Resistance
dc.relation.citationVolumeVol. 13
dc.relation.ispartofJournal of Global Antimicrobial Resistance, ISSN:22137165, Vol.13,(2018); pp. 254-260spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85047837671&doi=10.1016%2fj.jgar.2017.11.010&partnerID=40&md5=eb18b75718fef3ff95b64d8997474814spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAnidulafunginspa
dc.subject.keywordmasseng
dc.subject.keywordmatrix-assisted laser desorption-ionizationeng
dc.subject.keywordRibosome rnaspa
dc.subject.keywordAnidulafunginspa
dc.subject.keywordAntifungal agentspa
dc.subject.keywordCaspofunginspa
dc.subject.keywordEchinocandinspa
dc.subject.keywordAntifungal susceptibilityspa
dc.subject.keywordArticlespa
dc.subject.keywordBlood culturespa
dc.subject.keywordBroth dilutionspa
dc.subject.keywordCandidaspa
dc.subject.keywordCandida albicansspa
dc.subject.keywordCandida aurisspa
dc.subject.keywordCandida glabrataspa
dc.subject.keywordCandida haemuloniispa
dc.subject.keywordCandida intermediaspa
dc.subject.keywordCandida parapsilosisspa
dc.subject.keywordCandida tropicalisspa
dc.subject.keywordCandidemiaspa
dc.subject.keywordClavispora lusitaniaespa
dc.subject.keywordColombiaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordDna sequencespa
dc.subject.keywordEpsilometer testspa
dc.subject.keywordFks1 genespa
dc.subject.keywordFks2 genespa
dc.subject.keywordFungal genespa
dc.subject.keywordFungus identificationspa
dc.subject.keywordGene mutationspa
dc.subject.keywordGene sequencespa
dc.subject.keywordMatrix assisted laser desorption ionization time of flight mass spectrometryspa
dc.subject.keywordMeyerozyma guilliermondiispa
dc.subject.keywordMinimum inhibitory concentrationspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPichia kudriavzeviispa
dc.subject.keywordPriority journalspa
dc.subject.keywordSilent mutationspa
dc.subject.keywordSingle nucleotide polymorphismspa
dc.subject.keywordBlood culturespa
dc.subject.keywordCandidaspa
dc.subject.keywordCandidemiaspa
dc.subject.keywordComparative studyspa
dc.subject.keywordDisk diffusionspa
dc.subject.keywordDrug effectspa
dc.subject.keywordGeneticsspa
dc.subject.keywordHigh throughput sequencingspa
dc.subject.keywordHospitalspa
dc.subject.keywordHumanspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordMatrix-assisted laser desorption-ionization mass spectrometryspa
dc.subject.keywordMicrobial sensitivity testspa
dc.subject.keywordRna genespa
dc.subject.keywordAnidulafunginspa
dc.subject.keywordAntifungal agentsspa
dc.subject.keywordBlood culturespa
dc.subject.keywordCandidaspa
dc.subject.keywordCandidemiaspa
dc.subject.keywordCaspofunginspa
dc.subject.keywordColombiaspa
dc.subject.keywordDisk diffusion antimicrobial testsspa
dc.subject.keywordEchinocandinsspa
dc.subject.keywordGeneseng
dc.subject.keywordHigh-throughput nucleotide sequencingspa
dc.subject.keywordHospitalsspa
dc.subject.keywordHumansspa
dc.subject.keywordMicrobial sensitivity testsspa
dc.subject.keywordSpectrometryeng
dc.subject.keywordCandidaspa
dc.subject.keywordCandidaemiaspa
dc.subject.keywordEchinocandin susceptibilityspa
dc.subject.keywordFks silent mutationsspa
dc.subject.keywordMaldi-tof/msspa
dc.subject.keywordMolecular identificationspa
dc.titleComparative study of Candida spp. isolates: Identification and echinocandin susceptibility in isolates obtained from blood cultures in 15 hospitals in Medellín, Colombiaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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