Ítem
Acceso Abierto
Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients
Título de la revista
Autores
Ramírez, Juan David
Herrera, Giovanny
Muskus, Carlos
Méndez-Sarmiento, Claudia
Duque, María Clara
Butcher, Robert
Archivos
Fecha
2019
Directores
ISSN de la revista
Título del volumen
Editor
Elsevier B.V.
Buscar en:
Métricas alternativas
Resumen
Abstract
Aim: Here, we evaluate the ddPCR platform using an evaluated qPCR-based diagnostic assay for the detection of Leishmania infection in Cutaneous Leishmaniasis patients. Methods: A standard curve of cultured Leishmania parasite material and clinical samples of CL patients were tested with ddPCR to determine the analytical and diagnostic performance. Results: The limit of detection of the assay on the ddPCR platform was much higher than the published limit of detection of the same assay on the qPCR platform (100 vs 1 parasites/mL, respectively). Conclusion: While the performance of this assay in ddPCR format was acceptable for research purposes, it is not sufficient for clinical diagnostic purposes. The assay is more suited to the qPCR platform. © 2018 The Author(s)
Palabras clave
Keywords
Protozoal DNA , Protozoal DNA , Article , Droplet digital polymerase chain reaction , Human , Leishmania , Leishmania braziliensis , Leishmania donovani , Leishmania guyanensis , Leishmania infantum , Leishmania major , Leishmania mexicana , Leishmania panamensis , Limit of detection , Microorganism detection , Molecular pathology , Skin leishmaniasis , Task performance , Validation study , Evaluation study , Female , Genetics , Isolation and purification , Leishmania , Parasitology , Procedures , Real time polymerase chain reaction , Sensitivity and specificity , Skin leishmaniasis , DNA , Female , Humans , Leishmania , Leishmaniasis , Real-Time Polymerase Chain Reaction , Sensitivity and Specificity , Cutaneous Leishmaniasis , Ddpcr , Leishmania , Molecular diagnosis
