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Passive transfer of narcolepsy: Anti-TRIB2 autoantibody positive patient IgG causes hypothalamic orexin neuron loss and sleep attacks in mice

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Katzav, Aviva
Arango, Maria T.
Kivity, Shaye
Tanaka, Susumu
Givaty, Gili
Agmon-Levin, Nancy
Honda, Makoto
Anaya, Juan-Manuel
Chapman, Joab
Shoenfeld, Yehuda



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Narcolepsy is a sleep disorder characterized by excessive daytime sleepiness and cataplexy (a sudden weakening of posture muscle tone usually triggered by emotion) caused by the loss of orexin neurons in the hypothalamus. Autoimmune mechanisms are implicated in narcolepsy by increased frequency of specific HLA alleles and the presence of specific autoantibody (anti-Tribbles homolog 2 (TRIB2) antibodies) in the sera of patients with narcolepsy. Presently, we passively transferred narcolepsy to naïve mice by injecting intra-cerebra-ventricularly (ICV) pooled IgG positive for anti-TRIB2 antibodies. Narcolepsy-IgG-injected mice had a loss of the NeuN (neuronal marker), synaptophysin (synaptic marker) and orexin-positive neurons in the lateral hypothalamus area in narcolepsy compared to control-IgG-injected mice and these changes were associated with narcolepsy-like immobility attacks at four weeks post injection and with hyperactivity and long term memory deficits in the staircase and novel object recognition tests. Similar behavioral and cognitive deficits are observed in narcoleptic patients. This is the first report of passive transfer of experimental narcolepsy to naïve mice induced by autoantibodies and supports the autoimmune pathogenesis in narcolepsy. © 2013 Elsevier Ltd.
Palabras clave
Autoantibody , passive , inbred c3h , Biological marker , animal , physiological , Hla antibody , Immunoglobulin g , Neuron specific nuclear protein , Orexin , Synaptophysin , Trib2 autoantibody , Unclassified drug , Animal experiment , Animal model , Animal tissue , Article , Autoimmunity , Brain region , Cataplexy , Cognitive defect , Controlled study , Daytime somnolence , Human , Hyperactivity , Hypothalamus , Immobilization , Long term memory , Mouse , Narcolepsy , Nonhuman , Normal human , Passive transport , Pathogenesis , Priority journal , Recognition , Sleep , Anti-tribbles homolog 2 (trib2) antibodies , Behavioral deficits , Narcolepsy , Orexin , Passive transfer , Animals , Autoantibodies , Autoantigens , Cataplexy , Cell death , Disease models , Female , Humans , Hypothalamus , Immunization , Immunoglobulin g , Intracellular signaling peptides and proteins , Mice , Mice , Narcolepsy , Neurons , Neuropeptides , Pattern recognition , Anti-tribbles homolog 2 (trib2) antibodies , Behavioral deficits , Narcolepsy , Orexin , Passive transfer