Ítem
Solo Metadatos
Comparison of parasite loads in serum and blood samples from patients in acute and chronic phases of Chagas disease
Título de la revista
Autores
Hernández C.
Teherán A.
Flórez C.
Ramírez, Juan David
Fecha
2018
Directores
ISSN de la revista
Título del volumen
Editor
Cambridge University Press
Buscar en:
Métricas alternativas
Resumen
Abstract
Molecular methods have been developed for the detection and quantification of Trypanosoma cruzi DNA in blood samples from patients with Chagas disease. However, aspects of sample processing necessary for quantitative real-time PCR (qPCR), such as the addition of guanidine hydrochloride to whole blood samples, may limit timely access to molecular diagnosis. We analysed 169 samples from serum and guanidine-EDTA blood (GEB) obtained from patients in acute and chronic phases of Chagas disease. We applied qPCR targeted to the satellite DNA region. Finally, we compared the parasite loads and cycle of threshold values of the qPCR. The results confirmed the usefulness of serum samples for the detection and quantification of parasite DNA in patients with Chagas disease, especially in the acute phase. However, the parasite loads detected in serum samples from patients in the chronic phase were lower than those detected in GEB samples. The epidemiological implications of the findings are herein discussed. © Cambridge University Press 2018.
Palabras clave
Keywords
Enalapril maleate , Guanidine , Satellite dna , Acute disease , Adult , Article , Blood sampling , Chagas disease , Chronic disease , Comparative study , Female , Human , Limit of quantitation , Major clinical study , Male , Molecular diagnosis , Parasite load , Parasitemia , Polymerase chain reaction , Priority journal , Quantitative analysis , Real time polymerase chain reaction , Simulation , Dna , Qpcr , Satellite dna , Trypanosoma cruzi
