Ítem
Acceso Abierto
Activation of the PI3K/AKT signalling pathway by insulin-like growth factor type II stimulates metalloproteinase 9 mRNA expression in choriocarcinoma cells
Título de la revista
Autores
Pinzón M.L.
Díaz L.E.
Ortiz B.L.
Umaña A.
de Rodríguez S.C.
de Gómez M.S.
Archivos
Fecha
2009
Directores
ISSN de la revista
Título del volumen
Editor
Buscar en:
Métricas alternativas
Resumen
Abstract
Metalloproteinases 2 and 9 (MMP-2 and MMP-9) and Tissue Inhibitor-1 of MMPs (TIMP-1) could contribute to regulate the invasive behaviour of trophoblastic cells, effect which could be mediated by Insulin- like growth factor type II (IGF-II), which regulates the development and function of trophoblast at the foetal-maternal interface. The aim of this study was to investigate the role of the PI3K/AKT signalling pathway activated by IGF-II in the expression of MMP-2 and MMP-9 involved in the invasion process in the human choriocarcinoma cell line JEG-3. MMP-2, MMP-9 and TIMP-1mARNexpression in those cells were evaluated by RT-PCR using different IGF-II doses and conditions while signalling pathway was evaluated using Western blot. It was found that IGF-II does not contribute to trophoblastic cells proliferation, however, it promotes mARN expression of MMP-9 and TIMP-1, but not of MMP-2, in a dose-dependent way. The effect on MMP-9 expression is mediated through IGF-II activation of PI3K/AKT signaling pathway after phosphorylation of the IGF-I receptor (IGF-IR). According with the results a model is proposed in which the interaction of IGF-II with IGF-IR leads to PI3K/AKT activation and subsequent expression and activation of MMP-9. This activation is an essential requirement for the invasive process.
Palabras clave
Keywords
Invasion , Metalloproteinases , PI3K/AKT , Trophoblast
