Ítem
Solo Metadatos
Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model
Título de la revista
Autores
Yandar, Nubia
Pastorin, Giorgia
Prato, Maurizio
Bianco, Alberto
Patarroyo, Manuel Elkin
Lozano, José Manuel
Resumen
Abstract
We have covalently conjugated an N-terminus Plasmodium vivax apical membrane antigen-1 (AMA-1) peptide to functionalized carbon nanotubes (f-CNT). Immunological characterization of this molecular conjugate revealed that the immunogen-AMA-1 peptide was appropriately presented after being conjugated to CNTs as well as being recognized by BALB/c polyclonal antibodies. Subsequent experiments lead us to assess the AMA-1 peptide alone, as well as the CNT-peptide conjugate regarding rodent malarial infection. Remarkably, the peptide effectively controlled and delayed Plasmodium berghei-challenged animals' parasitaemia. The peptide-CNT conjugate displayed similar immunological properties to the peptide alone by protecting or delaying malarial infection. The peptide presentation by f-CNT to the immune system thus constitutes a promising approach for synthetic malarial vaccine formulation since the immunogen peptide conformation is well preserved. © 2008 Elsevier Ltd. All rights reserved.
Palabras clave
Keywords
Apical membrane antigen 1 , protozoan , Drug carrier , Polyclonal antibody , Amino terminal sequence , Animal experiment , Animal model , Article , Bagg albino mouse , Conjugation , Controlled study , Drug delivery system , Drug structure , Female , Immune system , Immunogenicity , Malaria , Mouse , Nonhuman , Parasitemia , Plasmodium berghei , Plasmodium vivax , Priority journal , Alleles , Amino acid sequence , Animals , Antigens , Blotting , Cytokines , Drug delivery systems , Enzyme-linked immunosorbent assay , Female , Fluorescent antibody technique , Genes , Hla-dr antigens , Malaria , Malaria vaccines , Membrane proteins , Mice , Mice , Microscopy , Molecular sequence data , Nanotubes , Plasmodium berghei , Plasmodium vivax , Protozoan proteins , Vaccines , Apical membrane antigen 1 (ama-1) , Functionalized carbon nanotubes (f-cnt) , Synthetic vaccine delivery system
