Ítem
Acceso Abierto
Functional characterization of Mycobacterium tuberculosis Rv2969c membrane protein
Título de la revista
Autores
Patarroyo, Manuel A.
Plaza, David F.
Ocampo, Marisol
Curtidor, Hernando
Forero, Martha
Rodriguez, Luis E.
Patarroyo, Manuel E.
Resumen
Abstract
Identifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra, and M. bovis BCG. Rabbits immunized with synthetic peptides from highly specific conserved regions of this protein produced antibodies recognizing 27 and 29 kDa bands in M. tuberculosis lysate, which is consistent with the molecular weight of the Rv2969c gene product in M. tuberculosis H37Rv. Immunoelectron microscopy revealed the protein was localized on the bacillus surface. Four and three specific high activity binding peptides (HABPs) to the A549 alveolar epithelial and U937 monocyte cell lines were found, respectively. Two of the HABPs found inhibited M. tuberculosis invasion of A549 cells, suggesting that these peptides might be good candidates to be included in a multiepitopic, subunit-based anti-tuberculosis vaccine. © 2008 Elsevier Inc. All rights reserved.
Palabras clave
Keywords
Antibody , Isoleucyllysylglutamylisoleucylvalylglycylaspartylvalylprolyl glycylisoleucylaspartylserylalanylalanylalanylthreonylalanyl threonylseryltyrosine , Membrane protein , Peptide , Phenylalanyltyrosylisoleucylvalylthreonylserylarginylaspartyl aspartyllysyllysylaspartylglycylvalylalanylglycylprolylglycylaspartylalanine , Synthetic peptide , Animal experiment , Article , Controlled study , Gene sequence , Human , Human cell , Immunization , Immunoelectron microscopy , Lung alveolus epithelium , Monocyte , Mycobacterium tuberculosis , Nonhuman , Nucleotide sequence , Polymerase chain reaction , Priority journal , Rabbit , Amino acid sequence , Animals , Antibodies , Bacterial proteins , Cell line , Genes , Humans , Membrane proteins , Microscopy , Molecular sequence data , Mycobacterium tuberculosis , Peptides , Rabbits , Tuberculosis vaccines , Mycobacterium tuberculosis , Oryctolagus cuniculus , High activity binding peptide (habp) , Mycobacterium tuberculosis-host cell interaction , Rv2969c
