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Specific interaction between mycobacterium tuberculosis lipoprotein-derived peptides and target cells inhibits Mycobacterial entry in vitro
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Ocampo, Marisol
Curtidor, Hernando
Vanegas, Magnolia
Patarroyo, Manuel A.
Patarroyo, Manuel E.
Fecha
2014
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Blackwell Publishing Ltd
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Abstract
Tuberculosis (TB) continues being one of the diseases having the greatest mortality rates around the world, 8.7 million cases having been reported in 2011. An efficient vaccine against TB having a great impact on public health is an urgent need. Usually, selecting antigens for vaccines has been based on proteins having immunogenic properties for patients suffering TB and having had promising results in mice and non-human primates. Our approach has been based on a functional approach involving the pathogen-host interaction in the search for antigens to be included in designing an efficient, minimal, subunit-based anti-TB vaccine. This means that Mycobacterium tuberculosis has mainly been involved in studies and that lipoproteins represent an important kind of protein on the cell envelope which can also contribute towards this pathogen's virulence. This study has assessed the expression of four lipoproteins from M. tuberculosis H37Rv, that is, Rv1411c (LprG), Rv1911c (LppC), Rv2270 (LppN) and Rv3763 (LpqH), and the possible biological activity of peptides derived from these. Five peptides were found for these proteins which had high specific binding to both alveolar A549 epithelial cells and U937 monocyte-derived macrophages which were able to significantly inhibit mycobacterial entry to these cells in vitro. © 2014 John Wiley and Sons A/S.
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Keywords
BCG vaccine , Tumor , Mycobacterium tuberculosis lipoprotein Rv1411c , Mycobacterium tuberculosis lipoprotein Rv1911c , Mycobacterium tuberculosis lipoprotein Rv2270 , Mycobacterium tuberculosis lipoprotein Rv3763 , Unclassified drug , Antibody , Bacterial protein , Lipoprotein , Microsphere , Peptide , Protein binding , A549 cell line , Amino acid sequence , Article , Bacterial membrane , Bacterial virulence , Controlled study , Epithelium cell , Host pathogen interaction , Human , In vitro study , Mycobacterium tuberculosis , Nonhuman , Protein expression , Protein localization , Protein structure , Target cell , U937 cell line , Biology , Chemistry , Host pathogen interaction , Immunology , Metabolism , Molecular genetics , Mycobacterium tuberculosis , Protein secondary structure , Synthesis , Tumor cell line , Mycobacterium tuberculosis , Amino Acid Sequence , Antibodies , Bacterial Proteins , Cell Line , Computational Biology , Host-Pathogen Interactions , Humans , Lipoproteins , Microspheres , Molecular Sequence Data , Mycobacterium tuberculosis , Peptides , Protein Binding , Protein Structure , U937 Cells , Antituberculosis vaccine , High activity binding peptide , Multi-epitope vaccine , Mycobacterium tuberculosis , Synthetic peptide
