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Lack of association between TNF-308 polymorphism and the clinical and immunological characteristics of systemic lupus erythematosus and primary Sjögren's syndrome
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Tobón G.J.
Correa P.A.
Gomez L.M.
Anaya, Juan-Manuel
Fecha
2005
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Abstract
Objective: To investigate the previously reported association of tumor necrosis factor alpha (TNF) -308 single nucleotide polymorphism (SNP) with the clinical course and immunological features in patients with systemic lupus erythematosus (SLE) and primary Sjögren's syndrome (pSS). Methods: The studied group consisted of 113 consecutive SLE and 65 pSS patients. TNF -308 SNP was determined by the polymerase chain reaction-restriction fragment length polymorphism technique. Clinical and immunological characteristics were assessed according to a standard protocol that included disease activity (SLEDAI) and damage (SLICC Damage Index). Serum TNF? levels were measured in samples collected from 32 patients with SLE and 16 with pSS by enzyme-linked immunosorbent assay. Results: The TNF2 allele (A) was observed in 46% and 54% of SLE and pSS patients, respectively. We failed to find any significant association between the -308 SNP and disease manifestations, the presence of autoantibodies or cytokine levels in either group. Conclusion: TNF -308 SNP (TNF2) does not exhibit a significant influence on the disease course or immunological response in SLE and pSS. Other genetic and/or environmental factors seem to be required and to be more important than TNF2 allele for the progression of these diseases.
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Autoantibody , systemic , Tumor necrosis factor , single nucleotide , Adult , Allele , Article , Blood sampling , Clinical feature , Clinical protocol , Controlled study , Disease activity , Disease course , Environmental factor , Enzyme linked immunosorbent assay , Female , Genetic analysis , Human , Immune response , Immunology , Major clinical study , Male , Polymerase chain reaction , Priority journal , Protein analysis , Protein blood level , Restriction fragment length polymorphism , Single nucleotide polymorphism , Sjoegren syndrome , Systemic lupus erythematosus , Adult , Enzyme-linked immunosorbent assay , Female , Genetic predisposition to disease , Health status , Humans , Lupus erythematosus , Male , Polymorphism , Severity of illness index , Sjogren's syndrome , Tumor necrosis factor-alpha , Polymorphism , Primary sjögren's syndrome , Systemic lupus erythematosus , Tumor necrosis factor
