"Functional, biochemical and 3D studies of Mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine"
"Tuberculosis (TB) is an air-born, transmissible disease, having an estimated 9.4 million new TB cases worldwide in 2009. Eventual control of this disease by developing a safe and efficient new vaccine able to detain its spread will have an enormous impact on public health policy. Selecting potential antigens to be included in a multi-epitope, minimal subunit-based, chemically-synthesized vaccine containing the minimum sequences needed for blocking mycobacterial interaction with host cells is a complex task due to the multiple mechanisms involved in M. tuberculosis infection and the mycobacterium's immune evasion mechanisms. Our methodology, described here takes into account a highly robust, specific, sensitive and functional approach to the search for potential epitopes to be included in an anti-TB vaccine; it has been based on identifying short mycobacterial protein fragments using synthetic peptides having high affinity interaction with alveolar epithelial cells (A549) and monocyte-derived macrophages (U937) which are able to block the microorganism's entry to target cells in in vitro assays. This manuscript presents a review of the results obtained with some of the MTB H37Rv proteins studied to date, aimed at using these high activity binding peptides (HABPs) as platforms to be included in a minimal subunit-based, multiepitope, chemically-synthesized, antituberculosis vaccine. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted."
Bacterial antigen ; Bacterial protein ; Bcg vaccine ; Rv 0180 c protein ; Rv 0227 c protein ; Rv 0679 c protein ; Rv 1490 protein ; Rv 1510 c protein ; Rv 1980 c protein ; Rv 2004 c protein ; Rv 2301 protein ; Rv 2536 protein ; Rv 2560 protein ; Rv 2707 protein ; Rv 2969c protein ; Rv 3166 c protein ; Rv 3481 c protein ; Rv 3629 c protein ; Rv 3630 protein ; Rv 3804 c protein ; Rv 3910 protein ; Synthetic peptide ; Unclassified drug ; Bioinformatics ; Immune response ; Molecular biology ; Mycobacterium tuberculosis ; Nonhuman ; Priority journal ; Protein analysis ; Protein binding ; Protein function ; Proteomics ; Review ; Three dimensional imaging ; Antigens, bacterial ; Bacterial adhesion ; Bacterial proteins ; Cell line ; Epithelial cells ; Humans ; Macrophages ; Mycobacterium tuberculosis ; Tuberculosis vaccines ; Mycobacterium tuberculosis surface protein ; Receptor-ligand interaction ; Subunit-vaccine ; Synthetic-peptides ;
- Artículos