Ítem
Solo Metadatos

Functional, biochemical and 3D studies of Mycobacterium tuberculosis protein peptides for an effective anti-tuberculosis vaccine

Título de la revista
Autores
Ocampo, Marisol
Patarroyo, Manuel A.
Vanegas, Magnolia
Alba, Martha P.
Patarroyo, Manuel E.

Fecha
2014

Directores

ISSN de la revista
Título del volumen
Editor

Buscar en:

Métricas alternativas

Resumen
Abstract
Tuberculosis (TB) is an air-born, transmissible disease, having an estimated 9.4 million new TB cases worldwide in 2009. Eventual control of this disease by developing a safe and efficient new vaccine able to detain its spread will have an enormous impact on public health policy. Selecting potential antigens to be included in a multi-epitope, minimal subunit-based, chemically-synthesized vaccine containing the minimum sequences needed for blocking mycobacterial interaction with host cells is a complex task due to the multiple mechanisms involved in M. tuberculosis infection and the mycobacterium's immune evasion mechanisms. Our methodology, described here takes into account a highly robust, specific, sensitive and functional approach to the search for potential epitopes to be included in an anti-TB vaccine; it has been based on identifying short mycobacterial protein fragments using synthetic peptides having high affinity interaction with alveolar epithelial cells (A549) and monocyte-derived macrophages (U937) which are able to block the microorganism's entry to target cells in in vitro assays. This manuscript presents a review of the results obtained with some of the MTB H37Rv proteins studied to date, aimed at using these high activity binding peptides (HABPs) as platforms to be included in a minimal subunit-based, multiepitope, chemically-synthesized, antituberculosis vaccine. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted.
Palabras clave
Keywords
Bacterial antigen , Bacterial protein , Bcg vaccine , Rv 0180 c protein , Rv 0227 c protein , Rv 0679 c protein , Rv 1490 protein , Rv 1510 c protein , Rv 1980 c protein , Rv 2004 c protein , Rv 2301 protein , Rv 2536 protein , Rv 2560 protein , Rv 2707 protein , Rv 2969c protein , Rv 3166 c protein , Rv 3481 c protein , Rv 3629 c protein , Rv 3630 protein , Rv 3804 c protein , Rv 3910 protein , Synthetic peptide , Unclassified drug , Bioinformatics , Immune response , Molecular biology , Mycobacterium tuberculosis , Nonhuman , Priority journal , Protein analysis , Protein binding , Protein function , Proteomics , Review , Three dimensional imaging , Antigens , Bacterial adhesion , Bacterial proteins , Cell line , Epithelial cells , Humans , Macrophages , Mycobacterium tuberculosis , Tuberculosis vaccines , Mycobacterium tuberculosis surface protein , Receptor-ligand interaction , Subunit-vaccine , Synthetic-peptides
Buscar en:
Colecciones