Delayed-type hypersensitivity and lymphocyte proliferation in response to leishmania major infection in a group of children in jericho

Alvarado , Ricardo; Enk, Claes; Jabel, Kamal; Schnur, Lionel; Frankenburg, Shoshana

doi:https://doi.org/10.1016/0035-9203(89)90637-8

Ítem

Solo Metadatos

Delayed-type hypersensitivity and lymphocyte proliferation in response to leishmania major infection in a group of children in jericho

https://repository.urosario.edu.co/handle/10336/25929
https://doi.org/10.1016/0035-9203(89)90637-8

Autores

Alvarado , Ricardo

Enk, Claes

Jabel, Kamal

Schnur, Lionel

Frankenburg, Shoshana

Fecha

1989-04

Editor

Elsevier

Buscar en:

Métricas alternativas

Abstract

The cellular response to Leishmania major was evaluated in vitro with a lymphocyte proliferation microtest, performed on 100 ?l of whole blood obtained by finger prick. The maximum time and optimum conditions for storage of fresh blood before testing were determined, and the ability of the assay to evaluate cellular immunity to Leishmania was compared to that of the classical Montenegro skin test. A positive correlation between the diameter of the skin induration and the stimulation index was demonstrated. Defining a positive skin test by induration ?5 mm, and a positive proliferation assay by a stimulation index >2·6 and a response ?3000 ct/min, we found a significant correlation between the 2 tests. The proliferation assay was less sensitive than the skin test, but somewhat more specific. Diagnostic specificities and sensitivities did not differ for the 2 tests.