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Long-term storage and safe retrieval of human papillomavirus DNA using FTA elute cards

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Bartha, Heidi
Morel, Adrien
Mougin, Christiane
Averous, Gerlinde
Legrain, Michèle
MurielFender

Fecha
2016-03-01

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Elsevier

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Abstract
Biobanking or collection and storage of specimens for future research purposes have become an essential tool in many fields of biomedical research and aims to provide a better understanding of disease mechanisms as well as the identification of disease-specific biomarkers that can navigate in complex diseases. In this study, we assessed the use of Flinders Technology Associates (FTA) cards as a long-term storage device for cervical specimens with suspected human papillomavirus (HPV) infections. HPV detection and genotyping results in liquid-based transport media were compared to HPV results from FTA cards. The overall agreement for the presence of any HPV infection between liquid-based medium and FTA cards stored for 1 year at ambient temperature was 100%. Reproducibility analysis of HPV detection and genotyping from FTA cards demonstrated that FTA cards are a reliable medium to store and preserve viral nucleic acids. Biobanking of cervical cells on FTA cards may provide a key resource for epidemiological and retrospective HPV studies.
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Keywords
Papillomavirus , Cervical cancer , Storage of cells , Biobanking
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